State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, China.
College of Agriculture and Animal Husbandry, Qinghai University, Xining, China.
PLoS One. 2018 Aug 13;13(8):e0202213. doi: 10.1371/journal.pone.0202213. eCollection 2018.
Sea buckthorn is one of the most important eco-economic tree species in China due to its ability to grow and produce acceptable yields under limited water and fertilizer availability. In this study, the differentially expressed genes under drought stress (DS) of sea buckthorn were identified and compared with control (CK) by RNA-Seq. A total of 122,803 unigenes were identified in sea buckthorn, and 70,025 unigenes significantly matched a sequence in at least one of the seven databases. A total of 24,060 (19.59%) unigenes can be assigned to 19 KEGG pathways, and 1,644 unigenes were differentially expressed between DS and CK, of which 519 unigenes were up-regulated and 1,125 unigenes down-regulated. Of the 47 significantly enriched GO terms, 14, 7 and 26 items were related to BP, CC and MF, respectively. KEGG enrichment analysis showed 398 DEGs involved in 97 different pathways, of which 119 DEGs were up-regulated and 279 DEGs were down-regulated under drought stress. In addition, we found 4438 transcriptor factors (TFs) in sea buckthorn, of which 100 were differentially expressed between DS and CK. These results lay a first foundation for further investigations of the very specific functions of these unigenes in sea buckthorn in response to drought stress.
沙棘是中国最重要的生态经济型树种之一，因为它能够在有限的水和肥料条件下生长并产生可接受的产量。在这项研究中，通过 RNA-Seq 鉴定了沙棘在干旱胁迫（DS）下的差异表达基因，并与对照（CK）进行了比较。在沙棘中鉴定出了 122803 个 unigenes，其中 70025 个 unigenes至少在 7 个数据库中的一个序列中显著匹配。共有 24060 个（19.59%）unigenes可以分配到 19 个 KEGG 途径，1644 个 unigenes在 DS 和 CK 之间差异表达，其中 519 个 unigenes上调，1125 个 unigenes下调。在 47 个显著富集的 GO 术语中，分别有 14、7 和 26 项与 BP、CC 和 MF 相关。KEGG 富集分析显示 398 个 DEGs 涉及 97 个不同的途径，其中 119 个 DEGs 上调，279 个 DEGs 下调。此外，我们在沙棘中发现了 4438 个转录因子（TFs），其中 100 个在 DS 和 CK 之间差异表达。这些结果为进一步研究这些在沙棘中对干旱胁迫响应的特定功能的 unigenes奠定了基础。
