钙离子对葡萄球菌α毒素诱导兔红细胞溶血的影响。
Effect of calcium ions on staphylococcal alpha-toxin-induced hemolysis of rabbit erythrocytes.
作者信息
Harshman S, Sugg N
出版信息
Infect Immun. 1985 Jan;47(1):37-40. doi: 10.1128/iai.47.1.37-40.1985.
Abstract
Calcium in millimolar concentrations protected rabbit erythrocytes from hemolysis caused by staphylococcal alpha-toxin. This effect was maximal at 30 mM CaCl2 and required the continued presence of calcium. The protection was not absolute and could be overcome by increased concentrations of alpha-toxin. Calcium did not block the binding of alpha-toxin to erythrocytes but inhibited the alpha-toxin-induced release of small ions from the cell as measured by 86Rb release. The transient removal of calcium was sufficient to abrogate its protective effect, suggesting that its action involves a reversible alteration in the state of the membrane. The three steps of the alpha-toxin-induced hemolytic sequence are: (i) binding to specific receptors, (ii) formation of transmembrane pores, and (iii) cell lysis. We concluded that calcium acted at step ii by impeding the lateral movement of alpha-toxin necessary to form the transmembrane hexamer pores.
摘要
毫摩尔浓度的钙可保护兔红细胞免受葡萄球菌α毒素引起的溶血。此效应在30 mM氯化钙时最大，且需要钙持续存在。这种保护并非绝对的，增加α毒素的浓度可克服这种保护作用。钙不会阻断α毒素与红细胞的结合，但可抑制α毒素诱导的小离子从细胞中释放（通过86Rb释放来测量）。短暂去除钙足以消除其保护作用，这表明其作用涉及膜状态的可逆改变。α毒素诱导的溶血过程的三个步骤为：（i）与特定受体结合，（ii）形成跨膜孔，以及（iii）细胞裂解。我们得出结论，钙在第二步起作用，通过阻碍形成跨膜六聚体孔所需的α毒素的横向移动。
相似文献
1
Effect of calcium ions on staphylococcal alpha-toxin-induced hemolysis of rabbit erythrocytes.钙离子对葡萄球菌α毒素诱导兔红细胞溶血的影响。
Infect Immun. 1985 Jan;47(1):37-40. doi: 10.1128/iai.47.1.37-40.1985.
2
HEMOLYSIS OF RABBIT ERYTHROCYTES BY PURIFIED STAPHYLOCOCCAL ALPHA-TOXIN. I. KINETICS OF THE LYTIC REACTION.纯化的葡萄球菌α毒素对兔红细胞的溶血作用。I. 溶血反应的动力学
J Bacteriol. 1964 Jan;87(1):127-35. doi: 10.1128/jb.87.1.127-135.1964.
3
Studies on the binding of staphylococcal 125I-labeled alpha-toxin to rabbit erythrocytes.
Biochemistry. 1976 Jun 1;15(11):2348-55. doi: 10.1021/bi00656a016.
4
Early events in the action of staphylococcal alpha-toxin on the plasma membrane of adrenocortical Y1 tumor cells.葡萄球菌α毒素作用于肾上腺皮质Y1肿瘤细胞质膜的早期事件。
Infect Immun. 1986 Sep;53(3):636-40. doi: 10.1128/iai.53.3.636-640.1986.
5
Effects of lectins on the hemolysis of rabbit erythrocytes by straphylococcal alpha toxin.
Microbiol Immunol. 1977;21(9):517-24. doi: 10.1111/j.1348-0421.1977.tb00317.x.
6
Staphylococcal alpha toxin promotes blood coagulation via attack on human platelets.葡萄球菌α毒素通过攻击人类血小板促进血液凝固。
J Exp Med. 1988 Aug 1;168(2):527-42. doi: 10.1084/jem.168.2.527.
7
Susceptibility to staphylococcal alpha-toxin of Friend virus-infected murine erythroblasts during differentiation.Friend病毒感染的小鼠成红细胞在分化过程中对葡萄球菌α毒素的敏感性
Infect Immun. 1985 Apr;48(1):114-8. doi: 10.1128/iai.48.1.114-118.1985.
8
HEMOLYSIS OF RABBIT ERYTHROCYTES BY PURIFIED STAPHYLOCOCCAL ALPHA-TOXIN. III. POTASSIUM RELEASE.纯化的葡萄球菌α毒素对兔红细胞的溶血作用。III. 钾离子释放
J Bacteriol. 1964 Jan;87(1):145-9. doi: 10.1128/jb.87.1.145-149.1964.
9
HEMOLYSIS OF RABBIT ERYTHROCYTES BY PURIFIED STAPHYLOCOCCAL ALPHA-TOXIN. II. EFFECTS OF INHIBITORS ON THE HEMOLYTIC SEQUENCE.纯化的葡萄球菌α毒素对兔红细胞的溶血作用。II. 抑制剂对溶血过程的影响。
J Bacteriol. 1964 Jan;87(1):136-44. doi: 10.1128/jb.87.1.136-144.1964.
10
Correlation between toxin binding and hemolytic activity in membrane damage by staphylococcal alpha-toxin.葡萄球菌α毒素致膜损伤中毒素结合与溶血活性之间的相关性
Infect Immun. 1984 Nov;46(2):318-23. doi: 10.1128/iai.46.2.318-323.1984.
引用本文的文献
1
Optimizing the Composition of Irrigation Fluid to Reduce the Potency of α-Toxin: Potential Role in the Treatment of Septic Arthritis.优化冲洗液成分以降低α毒素的效力：在脓毒性关节炎治疗中的潜在作用
Cartilage. 2020 Oct;11(4):500-511. doi: 10.1177/1947603518798888. Epub 2018 Sep 6.
2
Pharmacological characterization of cnidarian extracts from the Caribbean Sea: evaluation of anti-snake venom and antitumor properties.加勒比海刺胞动物提取物的药理学特性：抗蛇毒和抗肿瘤特性评估
J Venom Anim Toxins Incl Trop Dis. 2018 Aug 28;24:22. doi: 10.1186/s40409-018-0161-z. eCollection 2018.
3
Calcium-chelating alizarin and other anthraquinones inhibit biofilm formation and the hemolytic activity of Staphylococcus aureus.钙螯合茜素及其他蒽醌类物质可抑制金黄色葡萄球菌生物膜的形成及溶血活性。
Sci Rep. 2016 Jan 14;6:19267. doi: 10.1038/srep19267.
4
Structural representative of the protein family PF14466 has a new fold and establishes links with the C2 and PLAT domains from the widely distant Pfams PF00168 and PF01477.该蛋白质家族 PF14466 的结构代表具有新的折叠结构，并与广泛不同的 Pfam 家族 PF00168 和 PF01477 的 C2 和 PLAT 结构域建立联系。
Protein Sci. 2013 Jul;22(7):1000-7. doi: 10.1002/pro.2284. Epub 2013 Jun 6.
5
Effect of a zwitterionic surfactant (HPS) on the conformation and hemolytic activity of St I and St II, two isotoxins purified from Stichodactyla helianthus.两性离子表面活性剂（HPS）对从太阳海葵中纯化得到的两种同毒素St I和St II的构象及溶血活性的影响。
J Protein Chem. 2002 Aug;21(6):401-5. doi: 10.1023/a:1021130516229.
6
Staphylococcal alpha-toxin kills human keratinocytes by permeabilizing the plasma membrane for monovalent ions.葡萄球菌α毒素通过使质膜对单价离子通透来杀死人类角质形成细胞。
Infect Immun. 1993 Dec;61(12):4972-9. doi: 10.1128/iai.61.12.4972-4979.1993.
7
Use of a monoclonal antibody to determine the mode of transmembrane pore formation by streptolysin O.使用单克隆抗体确定链球菌溶血素O形成跨膜孔的方式。
Infect Immun. 1986 Dec;54(3):641-5. doi: 10.1128/iai.54.3.641-645.1986.
8
Early events in the action of staphylococcal alpha-toxin on the plasma membrane of adrenocortical Y1 tumor cells.葡萄球菌α毒素作用于肾上腺皮质Y1肿瘤细胞质膜的早期事件。
Infect Immun. 1986 Sep;53(3):636-40. doi: 10.1128/iai.53.3.636-640.1986.
9
Distribution of 3H-labeled staphylococcal alpha-toxin and a toxin fragment in mice.3H标记的葡萄球菌α毒素和一种毒素片段在小鼠体内的分布
Infect Immun. 1987 Aug;55(8):1906-13. doi: 10.1128/iai.55.8.1906-1913.1987.
10
Purification and characterization of Vibrio metschnikovii cytolysin.米氏弧菌溶细胞素的纯化与特性分析
Infect Immun. 1988 Apr;56(4):954-60. doi: 10.1128/iai.56.4.954-960.1988.
本文引用的文献
1
HEMOLYSIS OF RABBIT ERYTHROCYTES BY PURIFIED STAPHYLOCOCCAL ALPHA-TOXIN. III. POTASSIUM RELEASE.纯化的葡萄球菌α毒素对兔红细胞的溶血作用。III. 钾离子释放
J Bacteriol. 1964 Jan;87(1):145-9. doi: 10.1128/jb.87.1.145-149.1964.
2
Isolation and composition of staphylococcal alpha toxin.葡萄球菌α毒素的分离与组成
J Gen Microbiol. 1963 Mar;30:455-68. doi: 10.1099/00221287-30-3-455.
3
Membrane glycoprotein receptor and hole-forming properties of a cytolytic protein toxin.一种溶细胞蛋白毒素的膜糖蛋白受体及成孔特性
Biochemistry. 1982 Mar 30;21(7):1662-7. doi: 10.1021/bi00536a029.
4
Toxins of Staphylococcus aureus.金黄色葡萄球菌毒素
Pharmacol Ther. 1982;19(1):55-106. doi: 10.1016/0163-7258(82)90042-0.
5
Physical properties and function of phallolysin.阴茎溶素的物理性质与功能。
Biochemistry. 1983 Sep 13;22(19):4574-80. doi: 10.1021/bi00288a035.
6
Fusogenic mechanisms.融合机制。
Ciba Found Symp. 1984;103:28-44. doi: 10.1002/9780470720844.ch3.
7
The cytosol of human erythrocytes contains a highly Ca2+-sensitive thiol protease (calpain I) and its specific inhibitor protein (calpastatin).人类红细胞的胞质溶胶含有一种对钙离子高度敏感的硫醇蛋白酶（钙蛋白酶I）及其特异性抑制蛋白（钙蛋白酶抑制蛋白）。
J Biochem. 1981 Dec;90(6):1809-16. doi: 10.1093/oxfordjournals.jbchem.a133659.
8
Effects of drugs on calcium-related phenomena in red blood cells.
Fed Proc. 1981 Dec;40(14):2872-6.
9
On the mechanism of membrane damage by Staphylococcus aureus alpha-toxin.关于金黄色葡萄球菌α毒素导致膜损伤的机制
J Cell Biol. 1981 Oct;91(1):83-94. doi: 10.1083/jcb.91.1.83.
10
Studies on the binding of staphylococcal 125I-labeled alpha-toxin to rabbit erythrocytes.
Biochemistry. 1976 Jun 1;15(11):2348-55. doi: 10.1021/bi00656a016.
Zotero 插件