Alagna Adriana, Giacalone Vincenzo Maximiliano, Zenone Arturo, Martinez Marco, D'Anna Giovanni, Buffa Gaspare, Cavalca Caterina Jessica, Poli Anna, Varese Giovanna Cristina, Prigione Valeria Paola, Badalamenti Fabio
Department of Integrative Marine Ecology, Stazione Zoologica Anton Dohrn, Palermo, Italy.
National Biodiversity Future Centre (NBFC), Palermo, Italy.
Front Plant Sci. 2024 Nov 25;15:1433358. doi: 10.3389/fpls.2024.1433358. eCollection 2024.
Seed-based restoration methods are increasingly recognized as a relevant tool contributing to halt and reverse the loss of seagrass meadows while providing genetic and evolutionary benefit for the conservation of these habitats. protocols aimed at maximizing the survival of plantlets obtained from seeds in cultivation systems are therefore required. Previous trials of seedling culture of , the dominant seagrass of the Mediterranean Sea, recorded up to 40% loss due to mould development. In this study we aim to (i) identify the putative causal agents of seed decay and (ii) test the efficacy of copper sulphate (0.2 and 2 ppm) and of tannin-based products derived from chestnut, tara and quebracho in reducing seed and seedling decay, while assessing possible phytotoxic effects on plant development. and a putative new species were identified as possible causal agents of seed loss. The antimicrobial agents (copper and tannins) reduced seed contamination by 20%, although copper sulphate at 2 ppm strongly inhibited the root growth. Among tannins, chestnut and tara reduced seeds germination by up to 75% and decreased shoot and root development, while quebracho showed a less severe phytotoxic effect. The use of copper sulphate at 0.2 ppm is therefore recommended to prevent seedling loss in culture facilities since it reduces seed contamination with no phytotoxic effects. Our results contribute to improving the seedling culture of one the key species of the Mediterranean Sea, increasing propagule availability for restoration purposes.
基于种子的恢复方法越来越被认为是一种重要工具，有助于阻止和扭转海草草甸的丧失，同时为这些栖息地的保护提供遗传和进化益处。因此，需要制定旨在最大限度提高栽培系统中种子幼苗存活率的方案。此前对地中海优势海草进行的幼苗培养试验记录显示，由于霉菌滋生导致高达40%的损失。在本研究中，我们旨在：（i）确定种子腐烂的可能致病因子；（ii）测试硫酸铜（0.2和2 ppm）以及源自栗子、塔拉和柯伯栲的单宁基产品在减少种子和幼苗腐烂方面的功效，同时评估对植物发育可能产生的植物毒性作用。已确定一种假定的新物种以及另一种物种可能是种子损失的致病因子。抗菌剂（铜和单宁）使种子污染减少了20%，尽管2 ppm的硫酸铜强烈抑制根系生长。在单宁中，栗子和塔拉使种子发芽率降低多达75%，并减少地上部分和根系发育，而柯伯栲的植物毒性作用则不那么严重。因此，建议使用0.2 ppm的硫酸铜来防止栽培设施中的幼苗损失，因为它能减少种子污染且无植物毒性作用。我们的研究结果有助于改进地中海关键物种之一的幼苗培养，增加用于恢复目的的繁殖体数量。
