利用鲎试剂检测革兰氏阴性菌血症：在大鼠腹膜炎模型中的评估
Detection of gram-negative bacteremia by limulus amebocyte lysate assay: evaluation in a rat model of peritonitis.
作者信息
du Moulin G C, Lynch S E, Hedley-Whyte J, Broitman S A
出版信息
J Infect Dis. 1985 Jan;151(1):148-52. doi: 10.1093/infdis/151.1.148.
A spectrophotometric Limulus amebocyte lysate assay using lysis filtration and centrifugation has been developed for the detection of gram-negative bacteria in blood. The assay is directed at detection of endotoxin in viable and nonviable bacteria present in the blood-stream and not detection of free endotoxin in plasma. The assay was evaluated in a model of peritonitis in which rats were challenged with an inoculum consisting of sterilized human feces, barium sulfate, and one of eight species of bacteria. This assay was able to detect gram-negative bacteremia due to Escherichia coli, Pseudomonas aeruginosa, Serratia marcescens, Proteus mirabilis, and Klebsiella pneumoniae in the rat model when compared with sham-inoculated uninfected rats. The assay failed to detect bacteremia due to Bacteroides fragilis or Staphylococcus aureus, nor was there a significant rise in absorbance when a pellet containing sterilized feces was implanted in the rat.
已开发出一种使用裂解过滤和离心的分光光度法鲎试剂检测法，用于检测血液中的革兰氏阴性菌。该检测法旨在检测血流中活细菌和非活细菌中的内毒素，而非检测血浆中的游离内毒素。在腹膜炎模型中对该检测法进行了评估，在该模型中，用由灭菌人粪便、硫酸钡和八种细菌中的一种组成的接种物对大鼠进行攻击。与假接种未感染的大鼠相比，该检测法能够在大鼠模型中检测到由大肠杆菌、铜绿假单胞菌、粘质沙雷氏菌、奇异变形杆菌和肺炎克雷伯菌引起的革兰氏阴性菌血症。该检测法未能检测到脆弱拟杆菌或金黄色葡萄球菌引起的菌血症，并且当将含有灭菌粪便的沉淀物植入大鼠体内时，吸光度也没有显著升高。
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