A型基因多态性作为[具体对象]中头孢唑林接种物效应的决定因素
Polymorphisms in the type A gene as determinants of the cefazolin inoculum effect in .
作者信息
Lee Shinwon, Park Sohee, Kim Jeong Nam, Lee Soon Ok, Lee Jeong Eun, Lee Sun Hee
机构信息
Department of Internal Medicine, Pusan National University School of Medicine and Medical Research Institute, Pusan National University Hospital, Busan, Republic of Korea.
Department of Microbiology, College of Natural Sciences, Pusan National University, Busan, Republic of Korea.
出版信息
Antimicrob Agents Chemother. 2025 Jan 31;69(1):e0110624. doi: 10.1128/aac.01106-24. Epub 2024 Dec 10.
Plasmids containing the wild-type (WT) or mutant type A () gene (mutations at codons 226, 229, or both 226 and 229) were constructed and transformed into RN4220, yielding the strains WT-blaZ, M226-blaZ, M229-blaZ, and MB-blaZ. The high-inoculum cefazolin MIC was significantly lower in MB-blaZ and M226-blaZ than in WT-blaZ but not in M229-blaZ, suggesting that the single nucleotide polymorphism at codon 226 in contributes to the cefazolin inoculum effect.
构建了含有野生型（WT）或突变型A（密码子226、229或226和229均发生突变）基因的质粒，并将其转化到RN4220中，得到WT-blaZ、M226-blaZ、M229-blaZ和MB-blaZ菌株。MB-blaZ和M226-blaZ中高接种量头孢唑林的最低抑菌浓度（MIC）显著低于WT-blaZ，但M229-blaZ中并非如此，这表明A中密码子226处的单核苷酸多态性导致了头孢唑林接种量效应。
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