Zhu Xin-Yu, Gu Chun Tao
College of Life Sciences, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
Curr Microbiol. 2024 Dec 10;82(1):34. doi: 10.1007/s00284-024-04011-1.
Lactococcus muris was published in Cell Host & Microbe in November 2022 and validated in validation list no. 213 in October 2023. Lactococcus intestinalis was published in Antonie van Leeuwenhoek in May 2023 and validated in validation list no. 216 in March 2024. Both of them were isolated from the mouse gut. Previous studies indicated that L. muris DSM 109779 had the highest 16S rRNA gene sequence identity to Lactococcus garvieae (96.7%), and that L. intestinalis M2458 had the highest 16S rRNA gene sequence similarities to Lactococcus formosensis NBRC 109475 (97.6%) and L. garviae NBRC 100934 (97.1%). However, the taxonomic relationship between L. muris and L. intestinalis has not yet been investigated and therefore is the focus of the present study. In the present study, the relationship between L. muris and L. intestinalis was evaluated. The type strains of L. muris and L. intestinalis shared 100% 16S rRNA gene sequence similarity, 100% pheS sequence similarity, 100% rpoA sequence similarity, 99.9% average nucleotide identity (ANI) value and 98.5% digital DNA-DNA hybridization (dDDH) value, indicating that they represented the same species. On the basis of the results present here, we propose L. intestinalis Sun et al. 2024 as a later heterotypic synonym of L. muris Afrizal et al. 2023.
鼠源乳酸乳球菌于2022年11月发表于《细胞宿主与微生物》杂志，并于2023年10月在第213号验证列表中得到验证。肠道乳酸乳球菌于2023年5月发表于《安托尼·范·列文虎克》杂志，并于2024年3月在第216号验证列表中得到验证。它们均从小鼠肠道中分离得到。先前的研究表明，鼠源乳酸乳球菌DSM 109779与格氏乳酸乳球菌的16S rRNA基因序列同一性最高（96.7%），而肠道乳酸乳球菌M2458与台湾乳酸乳球菌NBRC 109475（97.6%）和格氏乳酸乳球菌NBRC 100934（97.1%）的16S rRNA基因序列相似性最高。然而，鼠源乳酸乳球菌和肠道乳酸乳球菌之间的分类关系尚未得到研究，因此是本研究的重点。在本研究中，对鼠源乳酸乳球菌和肠道乳酸乳球菌之间的关系进行了评估。鼠源乳酸乳球菌和肠道乳酸乳球菌的模式菌株16S rRNA基因序列相似性为100%，pheS序列相似性为100%，rpoA序列相似性为100%，平均核苷酸同一性（ANI）值为99.9%，数字DNA-DNA杂交（dDDH）值为98.5%，表明它们代表同一物种。基于此处给出的结果，我们提议将肠道乳酸乳球菌孙等人2024年的命名作为鼠源乳酸乳球菌阿夫里扎尔等人2023年命名的晚出异名。
