Yu Shiqiang, Li Liuxue, Zhao Huiying, Liu Ming, Jiang Linshu, Zhao Yuchao
Beijing Key Laboratory of Dairy Cow Nutrition, College of Animal Science and Technology, Beijing University of Agriculture, Beijing, China.
Beijing Beinong Enterprise Management Co., Ltd., Beijing, China.
Front Microbiol. 2023 Jun 9;14:1201262. doi: 10.3389/fmicb.2023.1201262. eCollection 2023.
Citrus flavonoid extracts (CFE) have the potential to reduce rumen inflammation, improve ruminal function, and enhance production performance in ruminants. Our previous studies have investigated the effects of CFE on the structure and function of rumen microbiota in dairy cows. However, it remains unclear whether CFE affects the prevalence of antibiotic resistance genes (ARG) and virulence factors genes (VFG) in the rumen. Therefore, metagenomics was used to identify the rumen ARG and VFG in lactating dairy cows fed with CFE diets. The results showed that CFE significantly reduced the levels of Multidrug and Antiphagocytosis in the rumen ( < 0.05) and increased the levels of Tetracycline, Iron uptake system, and Magnesium uptake system ( < 0.05). Furthermore, the changes were found to have associations with the phylum Lentisphaerae. It was concluded that CFE could be utilized as a natural plant product to regulate virulence factors and antibiotic resistance of rumen microbiota, thereby improving rumen homeostasis and the health of dairy cows.
柑橘类黄酮提取物（CFE）有潜力减轻瘤胃炎症、改善瘤胃功能并提高反刍动物的生产性能。我们之前的研究调查了CFE对奶牛瘤胃微生物群结构和功能的影响。然而，CFE是否会影响瘤胃中抗生素抗性基因（ARG）和毒力因子基因（VFG）的流行情况仍不清楚。因此，采用宏基因组学方法来鉴定饲喂CFE日粮的泌乳奶牛瘤胃中的ARG和VFG。结果表明，CFE显著降低了瘤胃中多药抗性和抗吞噬作用的水平（<0.05），并提高了四环素、铁摄取系统和镁摄取系统的水平（<0.05）。此外，发现这些变化与浮霉菌门有关。得出的结论是，CFE可作为一种天然植物产品来调节瘤胃微生物群的毒力因子和抗生素抗性，从而改善瘤胃内环境稳定和奶牛健康。
