Chen Dong, Ma Yilin, Yang Annan, Hu Liping, Zhou Hanlin, Xu Jun, Chen Shanze, Nie Dingmeng, Feng Weifeng, Cai Huaihong, Cong Yanguang, Pi Jiang, Rao Lang, Huang Xueqin, Sun Pinghua, Zhou Haibo
Institute for Safflower Industry Research, Key Laboratory of Xinjiang Phytomedicine Resource and Utilization (Ministry of Education), School of Pharmacy, Shihezi University, Shihezi, 832003, China.
Department of Urology, State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China.
Adv Sci (Weinh). 2025 Feb;12(5):e2411747. doi: 10.1002/advs.202411747. Epub 2024 Dec 10.
Prostate specific antigen (PSA) is widely used in liquid biopsy of prostate cancer (PCa) but still faces challenges due to the poor specificity. Herein, this study reports a double-SERS satellite immunoassay, made of an Au-Ag dealloyed intra-nanogap nanoflower (Au-Ag DINF) with strong SERS signals and Au magnetic nanoparticles (AuMNPs) with magnetic capture and SERS amplification, for sensing multiple PSA (free PSA (fPSA), complexed PSA (cPSA) and [-2]proPSA (p2PSA)) toward potential PCa screening. Unlike the previous studies focus on the tPSA and fPSA/tPSA ratio (f/t PSA%), this work introduces a multiple PSA-mediated Prostate Health Index (PHI) assay with significantly increased the predictive accuracy and specificity of PCa, especially the patients with a tPSA level in the "diagnostic gray zone". Practical analysis of clinical samples demonstrated the SERS immunoassay is capable of identifying the PCa subjects from healthy candidates (N = 13), and monitoring PCa patients before and after surgery (N = 5), as well as screening suspicious ones who are in the "gray zone" (N = 10). Overall, benefiting from the PSA-responsive satellite immuno-nanoassemble strategy, dual-SERS magnification effect, and multiple PSA-based PHI assessment, this proposes bioassay held enormous potential for the early diagnosis, screening, monitoring, and prognosis of PCa.
前列腺特异性抗原（PSA）广泛应用于前列腺癌（PCa）的液体活检，但由于特异性较差，仍面临挑战。在此，本研究报道了一种双表面增强拉曼散射（SERS）卫星免疫分析方法，该方法由具有强SERS信号的金-银脱合金化纳米间隙内纳米花（Au-Ag DINF）和具有磁捕获及SERS放大作用的金磁性纳米颗粒（AuMNPs）组成，用于检测多种PSA（游离PSA（fPSA）、复合PSA（cPSA）和[-2]前体PSA（p2PSA）），以进行潜在的PCa筛查。与以往专注于总PSA（tPSA）和fPSA/tPSA比值（f/t PSA%）的研究不同，本研究引入了一种多种PSA介导的前列腺健康指数（PHI）检测方法，显著提高了PCa的预测准确性和特异性，尤其是对于tPSA水平处于“诊断灰色地带”的患者。临床样本的实际分析表明，SERS免疫分析能够从健康受试者（N = 13）中识别出PCa患者，监测PCa患者手术前后的情况（N = 5），以及筛查处于“灰色地带”的可疑患者（N = 10）。总体而言，受益于PSA响应性卫星免疫纳米组装策略、双SERS放大效应以及基于多种PSA的PHI评估，这种生物检测方法在PCa的早期诊断、筛查、监测和预后方面具有巨大潜力。
