Jamwal Ashima, Varghese Gerlin, Tripathy Sarvodaya, Sarawat Deepika, Patel Sangram Singh, Kar Mitra, Tejan Nidhi, Sahu Chinmoy
Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India.
Am J Trop Med Hyg. 2024 Dec 10;112(3):539-543. doi: 10.4269/ajtmh.24-0421. Print 2025 Mar 5.
Ralstonia species are recently emerging as pathogens of human importance. This study was performed to assess the distribution of Ralstonia species among bacteremia patients and the clinical and demographical factors of these patients. This retrospective observational study was performed in the Microbiology Department of a tertiary care center in North India from April 2022 to April 2024. All in-patients with blood cultures positive for Ralstonia species for the first time during the study period were included in the study. Positive blood cultures were inoculated on blood and MacConkey agar. Identification was done by matrix-assisted laser desorption ionization-time of flight mass spectrometry, and antibiotic sensitivity testing was determined by disc diffusion assay. During the study period, 26 isolates of Ralstonia species were identified. R. mannitolilytica was the predominant isolate, followed by R. pickettii. Most patients with Ralstonia bacteremia belonged to the age group of <60 years (n = 20; 78%). The median age of the study population was 62 years. The male-to-female ratio was 1.6:1. Most of the patients presented with complaints of fever (77%), followed by epigastric pain (38.4%). Among the underlying comorbidities, most of the patients with Ralstonia bacteremia had sepsis (73%), followed by multiorgan dysfunction syndrome (30.7%). None of the R. mannitolilytica isolates showed sensitivity to imipenem, meropenem, aztreonam, and ceftazidime. On comparing both the isolates, R. pickettii showed better sensitivity to amikacin, imipenem, meropenem, aztreonam, and ceftazidime than R. mannitolilytica. Overall mortality of the patients in the study was 22% (n = 6).
嗜麦芽窄食单胞菌属最近逐渐成为具有重要医学意义的病原体。本研究旨在评估嗜麦芽窄食单胞菌属在菌血症患者中的分布情况以及这些患者的临床和人口统计学因素。这项回顾性观察研究于2022年4月至2024年4月在印度北部一家三级护理中心的微生物科进行。研究纳入了在研究期间首次血培养嗜麦芽窄食单胞菌属呈阳性的所有住院患者。将阳性血培养物接种于血琼脂和麦康凯琼脂上。通过基质辅助激光解吸电离飞行时间质谱进行鉴定，并通过纸片扩散法测定抗生素敏感性。在研究期间，共鉴定出26株嗜麦芽窄食单胞菌属菌株。嗜麦芽寡养单胞菌是主要分离株，其次是皮氏嗜麦芽窄食单胞菌。大多数嗜麦芽窄食单胞菌菌血症患者年龄小于60岁（n = 20；78%）。研究人群的中位年龄为62岁。男女比例为1.6:1。大多数患者表现为发热（77%），其次是上腹部疼痛（38.4%）。在基础合并症中，大多数嗜麦芽窄食单胞菌菌血症患者患有败血症（73%），其次是多器官功能障碍综合征（30.7%）。嗜麦芽寡养单胞菌分离株对亚胺培南、美罗培南、氨曲南和头孢他啶均无敏感性。比较两种分离株，皮氏嗜麦芽窄食单胞菌对阿米卡星、亚胺培南、美罗培南、氨曲南和头孢他啶的敏感性高于嗜麦芽寡养单胞菌。研究中患者的总体死亡率为22%（n = 6）。
