Sun Yuting, Cai Junhong, Zhang Yanan, Bao Shan
Department of Gynecology and Obstetrics Mudanjiang Medical University Affliated Honggi Hospital, No.5 Tongxiang Road, Aimin District, Mudanjiang,Heilongjiang 157011, China.
Medical Laboratory Center, Hainan General Hospital, Haikou 570102, China.
J Reprod Immunol. 2025 Feb;167:104414. doi: 10.1016/j.jri.2024.104414. Epub 2024 Dec 6.
We wished to ascertain if there is an association between neutrophil extracellular traps and endometriosis (EMS). We collected the lesional tissues and normal endometrium of 30 patients suffering from endometriosis. Samples were also taken from healthy controls. Blood from the peripheral circulation was collected to isolate serum and neutrophils. A mouse model of endometriosis was also created. Expression of citrullinated histone and the myeloperoxidase level in tissue were measured by immunofluorescence staining and western blotting. The myeloperoxidase level in peripheral blood serum was measured by enzyme-linked immunosorbent assay. Staining (Trypan Blue) and flow cytometry were used to measure the apoptosis of neutrophils in peripheral blood. BALB/C mice were modeled by allotransplantation, and the experimental parameters noted above quantified. The myeloperoxidase content in the peripheral blood of patients with endometriosis was increased compared with that in healthy controls. Flow cytometry showed that the percent apoptosis of neutrophils in patients with endometriosis was lower than that in healthy controls. Expression of citrullinated histone was higher in the endometriosis group in humans and mice compared with respective controls according to immunofluorescence staining and western blotting. Our data suggest that a high concentration of neutrophil extracellular traps was observed in humans and mice suffering from endometriosis.
我们希望确定中性粒细胞胞外诱捕网与子宫内膜异位症（EMS）之间是否存在关联。我们收集了30例子宫内膜异位症患者的病变组织和正常子宫内膜。还从健康对照者身上采集了样本。采集外周循环血液以分离血清和中性粒细胞。还建立了子宫内膜异位症小鼠模型。通过免疫荧光染色和蛋白质印迹法测量组织中瓜氨酸化组蛋白的表达和髓过氧化物酶水平。通过酶联免疫吸附测定法测量外周血血清中的髓过氧化物酶水平。使用染色（台盼蓝）和流式细胞术测量外周血中性粒细胞的凋亡。通过同种异体移植对BALB/C小鼠进行建模，并对上述实验参数进行量化。与健康对照者相比，子宫内膜异位症患者外周血中的髓过氧化物酶含量增加。流式细胞术显示，子宫内膜异位症患者中性粒细胞的凋亡百分比低于健康对照者。根据免疫荧光染色和蛋白质印迹法，与各自的对照相比，人类和小鼠子宫内膜异位症组中瓜氨酸化组蛋白的表达更高。我们的数据表明，在患有子宫内膜异位症的人类和小鼠中观察到高浓度的中性粒细胞胞外诱捕网。
