Hu Xiaopeng, Wang Ke, Yang Yufan, Ding Baomiao, Yu Chunqi
College of Life Science, Yangtze University, Jingzhou 434023, Hubei Province, PR China.
College of Life Science, Yangtze University, Jingzhou 434023, Hubei Province, PR China.
Food Chem. 2025 Mar 1;467:142387. doi: 10.1016/j.foodchem.2024.142387. Epub 2024 Dec 6.
This work aimed to develop a fluorescence/colorimetric sensor for the ultrasensitive detection of AFB1 based on the aptamers and molecular imprinted polymers (MIPs). The polydopamine on FeO (FeO@MIP) was used to achieve efficient separation of AFB1. The aptamer-modified carbon dots and metal-organic frameworks (Apt-CDs@MOF) were used to form sandwich particles (FeO@MIP-AFB1-Apt-CDs@MOF). The sandwich particles were separated and removed using magnets, and the remaining Apt-CDs@MOF in suspension was used for both fluorescence and colorimetric detection. The sensor reached the linear range of 0.05-150 ng mL (fluorescence channel) and 0.1-100 ng mL (colorimetric channel). The detection limit can be as low as 37.0 pg mL (fluorescence channel) and 13.0 pg mL (colorimetric channel). Through the clever use of sandwich structure, the sensor represents a novel method for interference-free detection of AFB1. The proposed sensor effectiveness was further validated by quantifying AFB1 in untreated edible oil, which shows great potential for application.
这项工作旨在基于适体和分子印迹聚合物（MIPs）开发一种用于超灵敏检测黄曲霉毒素B1（AFB1）的荧光/比色传感器。FeO上的聚多巴胺（FeO@MIP）用于实现AFB1的高效分离。适体修饰的碳点和金属有机框架（Apt-CDs@MOF）用于形成夹心颗粒（FeO@MIP-AFB1-Apt-CDs@MOF）。使用磁铁分离并去除夹心颗粒，悬浮液中剩余的Apt-CDs@MOF用于荧光和比色检测。该传感器在荧光通道的线性范围为0.05 - 150 ng/mL，在比色通道的线性范围为0.1 - 100 ng/mL。检测限可低至37.0 pg/mL（荧光通道）和13.0 pg/mL（比色通道）。通过巧妙利用夹心结构，该传感器代表了一种无干扰检测AFB1的新方法。通过对未处理食用油中的AFB1进行定量，进一步验证了所提出传感器的有效性，显示出巨大的应用潜力。
