Sun Xuyuan, Liu Hao, Dai Zhengyuan, Wang Ying, Li Li, Ding Yaping
Department of Chemistry, College of Sciences, Shanghai University, Shanghai, 200444, PR China.
Department of Chemistry, College of Sciences, Shanghai University, Shanghai, 200444, PR China.
Food Chem. 2025 Mar 30;469:142317. doi: 10.1016/j.foodchem.2024.142317. Epub 2024 Dec 4.
A molecularly imprinted fluorescent aptasensor was designed for selective detection of quinine (Qn) based on dual functional monomers. In the sol-gel polymerization of molecularly imprinted polymers (MIPs), 3-aminopropyltriethoxysilane (APTES) and quinine aptamer (Apt) were employed as dual functional monomers, and Qn was the template molecule. Near-infrared carbon dots (RCDs) were used as fluorescence signal probe, and effectively avoided the interference of fluorescence emitted by Qn. Due to the diverse interaction among APTES, Apt and Qn, the near-infrared fluorescent molecularly imprinted aptasensor (RCDs-Apt@MIPs) exhibited higher specificity towards Qn than the sensor with APTES or Apt as a single functional monomer. The sensor had a wide linear range of 5.0-110.0 μM with a low detection limit of 1.7 μM. Satisfactory recovery between 93.4% and 104.0% with relative standard deviation from 1.30% to 3.60% in drinks, which showed great potential that this method can be used to detect Qn in food.
基于双功能单体设计了一种用于选择性检测奎宁（Qn）的分子印迹荧光适配体传感器。在分子印迹聚合物（MIPs）的溶胶 - 凝胶聚合过程中，3 - 氨丙基三乙氧基硅烷（APTES）和奎宁适配体（Apt）被用作双功能单体，Qn为模板分子。近红外碳点（RCDs）用作荧光信号探针，有效避免了Qn发射荧光的干扰。由于APTES、Apt和Qn之间存在多种相互作用，近红外荧光分子印迹适配体传感器（RCDs - Apt@MIPs）对Qn的特异性高于以APTES或Apt作为单一功能单体的传感器。该传感器具有5.0 - 110.0 μM的宽线性范围，检测限低至1.7 μM。在饮料中的回收率在93.4%至104.0%之间，相对标准偏差为1.30%至3.60%，表明该方法在检测食品中Qn方面具有巨大潜力。
