Ge Yumei, Ji Youqi, Mei Jianhua, Zhang Maojun, Li Yumin, Ye Bifeng, Chen Honghu, Chen Xiuying
Department of Clinical Laboratory, Zhejiang Provincial People's Hospital (Affiliated People's Hospital, Hangzhou Medical College), Hangzhou, China.
School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou, China.
Foodborne Pathog Dis. 2024 Dec 10. doi: 10.1089/fpd.2024.0008.
is one of the leading causes of bacterial foodborne diarrheal diseases throughout the world. Reported outbreaks of are infrequent in China. This article described such an outbreak among students from a junior high school in East China during November 2019. A total of 40 samples were collected as follows: 24 stool samples from patients, 12 stool samples from kitchen staff members, and 4 water samples from cafeteria. The stool samples were tested for the presence of , enterotoxigenic , , , , , , , , , , , , , and by real-time polymerase chain reaction (PCR). Pulsed-field gel electrophoresis (PFGE), next-generation sequencing, and antimicrobial susceptibility testing were performed to determine the relatedness of the isolates in this outbreak. Forty-seven cases were reported with diarrhea among 569 seventh grade students and staff. In these 47 cases, we detected that through real-time PCR in 16 fecal samples was positive, whereas the remaining fecal samples were tested negative by real-time PCR. Only three strains were isolated from stool samples of case patients, and all of them showed 100% PFGE similarity to strain ST6913. Whole genome sequencing analysis revealed no single nucleotide variation in the three isolates. This is one of the few reports in China about outbreak caused by . ST6913 was responsible for this outbreak.
是全球细菌性食源性腹泻疾病的主要病因之一。在中国，报道的疫情并不常见。本文描述了2019年11月在中国东部一所初中学生中发生的此类疫情。共采集了40份样本，具体如下：24份患者粪便样本、12份厨房工作人员粪便样本和4份食堂水样。通过实时聚合酶链反应（PCR）检测粪便样本中是否存在、产肠毒素、、、、、、、、、、、、、和。进行脉冲场凝胶电泳（PFGE）、下一代测序和抗菌药物敏感性测试，以确定此次疫情中分离株的相关性。在569名七年级学生和工作人员中报告了47例腹泻病例。在这47例病例中，我们通过实时PCR检测发现16份粪便样本中的呈阳性，而其余粪便样本经实时PCR检测为阴性。仅从病例患者的粪便样本中分离出三株菌株，所有菌株与ST6913菌株的PFGE相似度均为100%。全基因组测序分析显示，这三株分离株没有单核苷酸变异。这是中国为数不多的关于由引起的疫情的报告之一。ST6913是此次疫情的病原体。
