Yang Mingfang, Wang Xin, Zheng Luping, Zhu Yongli
Department of Microbiology, Tonglu County Center for Disease Control and Prevention (Tonglu County Health Inspection Center), Hangzhou, China.
Medical Laboratory, Hangzhou First People's Hospital Tonglu Campus, Hangzhou, China.
Front Microbiol. 2025 Jun 23;16:1599555. doi: 10.3389/fmicb.2025.1599555. eCollection 2025.
() is a zoonotic pathogen and is the most prevalent foodborne pathogen globally. The increasing antimicrobial resistance and gene mutation pose a threat to public health and trigger us to enhance surveillance. This study illustrated the antimicrobial resistance profiles, virulence factors, and multilocus sequence typing (MLST) profiles of 47 isolates collected from human stool and raw poultry meat samples between 2022 and 2023 in Hangzhou, China. Notably, 88.9% (16/18) human-derived and 82.9% (24/29) poultry-derived displayed multidrug resistance (MDR) profiles, nalidixic acid + ciprofloxacin + tetracycline was prevalent among them. Relatively high rates of resistance to florfenicol were observed in , 38.9% (7/18) from human sources and 44.8% (13/29) from poultry sources. Comprehensive Antibiotic Resistance Database (CARD) and ResFinder database showed () was the dominant factor in quinolones resistance while a rare () was found in one poultry-derived . All 37 tetracycline-resistant strains harbored the gene. All 20 florfenicol-resistant did not have any related genes. Several key virulence factor genes associated with adherence (, and ), invasion ( and ), capsule biosynthesis/transport genes (, and ) and lipooligosaccharide (LOS) biosynthesis genes (, and ) were conserved in . MLST analysis revealed high genetic diversity, identifying 28 sequence types (STs), including 3 novel STs, 20 of which belonged to 10 clonal complexes (CCs), and 8 were unassigned. CC-464 and CC-21 strains carried the most virulence genes, correlating with clinical severity, yet represent a minority in local isolates. The most abundant CCs were CC-443 (21.3%, 10/47) and CC-574 (19.1%, 9/47), mainly isolated from poultry. These findings highlight that isolates in Hangzhou had high genetic diversity and MDR, CC-443 and CC-574 were the predominant strains. It is necessary to monitor human-poultry transmission and emerging resistance phenotypes.
（）是一种人畜共患病原体，是全球最普遍的食源性病原体。日益增加的抗菌药物耐药性和基因突变对公众健康构成威胁，并促使我们加强监测。本研究阐述了2022年至2023年期间在中国杭州从人类粪便和生禽肉样本中收集的47株分离株的抗菌药物耐药性概况、毒力因子和多位点序列分型（MLST）概况。值得注意的是，88.9%（16/18）的人类来源和82.9%（24/29）的家禽来源表现出多重耐药（MDR）概况，其中萘啶酸+环丙沙星+四环素较为普遍。在（）中观察到相对较高的氟苯尼考耐药率，人类来源的为38.9%（7/18），家禽来源的为44.8%（13/29）。综合抗生素耐药性数据库（CARD）和ResFinder数据库显示，（）是喹诺酮类耐药的主要因素，而在一株家禽来源的（）中发现了罕见的（）。所有37株四环素耐药（）菌株均携带（）基因。所有20株氟苯尼考耐药（）均未发现任何相关基因。与黏附（（）、（）和（））、侵袭（（）和（））、荚膜生物合成/转运基因（（）、（）和（））以及脂寡糖（LOS）生物合成基因（（）、（）和（））相关的几个关键毒力因子基因在（）中是保守的。MLST分析揭示了高度的遗传多样性，鉴定出28种序列类型（STs），包括3种新的STs，其中20种属于10个克隆复合体（CCs），8种未分配。CC-464和CC-21菌株携带的毒力基因最多，与临床严重程度相关，但在本地分离株中占少数。最丰富的CCs是CC-443（21.3%，10/47）和CC-574（19.1%，9/47），主要从家禽中分离得到。这些发现突出表明，杭州的（）分离株具有高度的遗传多样性和MDR，CC-443和CC-574是主要菌株。有必要监测人禽传播和新出现的耐药表型。
