迷走神经干切断术对大鼠胃肠道中5-羟色胺分布的影响。

The effect of truncal vagotomy on serotonin distribution in the rat gastrointestinal tract.

作者信息

Gomes G M, Dahlström A, Grimelius L, Johansson H, Ahlman H

出版信息

J Surg Res. 1985 Jan;38(1):13-6. doi: 10.1016/0022-4804(85)90004-6.

Abstract

The 5-HT levels in biopsies from the rat gastrointestinal tract were measured by a sensitive liquid chromatography method with electrochemical detection. The highest levels were found in the gastroduodenal and cecal regions. Sham-operated controls were compared with rats subjected to combined truncal vagotomy and pyloroplasty or pyloroplasty alone 5 weeks after surgery. Significantly decreased 5-HT levels were demonstrated in animals subjected to vagotomy + pyloroplasty or to pyloroplasty alone, compared with sham-operated controls. In the upper gastrointestinal tract of vagotomized animals significantly decreased 5-HT levels were found when compared with animals subjected to pyloroplasty alone. However, anesthesia and surgery (sham operation) per se markedly increased 5-HT levels of the gut even 5 weeks postoperatively, when compared with acutely killed rats. The underlying mechanism is still obscure. However, vagotomy largely prevented this increase. Therefore, the importance of correct controls is emphasized, since previous investigations claim to have demonstrated increased 5-HT levels in vagotomized animals, when compared with nonoperated controls.

摘要

采用灵敏的液相色谱电化学检测法测定大鼠胃肠道活检组织中的5-羟色胺（5-HT）水平。发现胃十二指肠和盲肠区域的水平最高。将假手术对照组与术后5周接受联合迷走神经干切断术和幽门成形术或仅接受幽门成形术的大鼠进行比较。与假手术对照组相比，接受迷走神经切断术+幽门成形术或仅接受幽门成形术的动物5-HT水平显著降低。与仅接受幽门成形术的动物相比，在迷走神经切断术动物的上胃肠道中发现5-HT水平显著降低。然而，与急性处死的大鼠相比，麻醉和手术（假手术）本身即使在术后5周也会显著提高肠道的5-HT水平。其潜在机制仍不清楚。然而，迷走神经切断术在很大程度上阻止了这种升高。因此，强调了正确设置对照组的重要性，因为先前的研究声称与未手术的对照组相比，已证明迷走神经切断术动物的5-HT水平升高。