Li Wenfeng, Yan Shengkun, Fu Xueqin, Tang Jingran, Yang Hongyan
School of Life Science and Biotechnology, Yangtze Normal University, Chongqing 408100, China.
Agricultural Mechanization Institute, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China.
Food Res Int. 2025 Jan;199:115348. doi: 10.1016/j.foodres.2024.115348. Epub 2024 Nov 19.
Six macroporous resins (MRs) were employed for the adsorption and desorption of neochlorogenic acid-enriched polyphenols from the European plum (NAPEP). X-5 exhibited the most significant neochlorogenic acid adsorption (99.62 %), desorption (44.80 %), and recovery (44.29 %) rates. The adsorption kinetics were described using a pseudo-second-order model while the Freundlich model indicated that X-5-adsorbing NAPEP was a spontaneous, exothermic process. NAPEP was effectively eluted from X-5 using a 60 % ethanol solution. Ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) revealed the presence of 21 polyphenol compounds in NAPEP, with chlorogenic acids accounting for 93 % of the overall composition. NAPEP demonstrated antioxidant efficacy comparable to chlorogenic acid. Furthermore, NAPEP administration effectively triggered apoptosis in A549 cells by producing reactive oxygen species, which regulated the AKT pathway. These results indicated that NAPEP could be utilized as an antioxidant and anti-cancer agent in functional foods.
六种大孔树脂用于从欧洲李中吸附和解吸富含新绿原酸的多酚（NAPEP）。X-5表现出最显著的新绿原酸吸附率（99.62%）、解吸率（44.80%）和回收率（44.29%）。吸附动力学用准二级模型描述，而弗伦德里希模型表明X-5吸附NAPEP是一个自发的放热过程。使用60%乙醇溶液可有效从X-5上洗脱NAPEP。超高效液相色谱-三重四极杆质谱联用（UHPLC-QqQ-MS/MS）显示NAPEP中存在21种多酚化合物，其中绿原酸占总成分的93%。NAPEP表现出与绿原酸相当的抗氧化功效。此外，NAPEP给药通过产生活性氧有效诱导A549细胞凋亡，从而调节AKT途径。这些结果表明，NAPEP可作为功能性食品中的抗氧化剂和抗癌剂。
