Li Huanhuan, Guo Fu, Zhao Yanlong, Wang Chaoli, Fan Ziwei, Feng Yajun, Ji Xiang, Tao Luna, Ma Chao, Qian Jiajun, Zhao Yue, Liu Qianwen, Sehgal Sunish K, Liu Cheng, Liu Wenxuan
The State Key Laboratory of Wheat and Maize Crop Science, College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, Henan, China.
Department of Agronomy, Horticulture and Plant Science, South Dakota State University, Brookings, SD, 57007, USA.
Theor Appl Genet. 2024 Dec 10;138(1):4. doi: 10.1007/s00122-024-04792-w.
A physical map of Aegilops geniculata chromosome 7M was constructed, and a novel purple coleoptile gene was localized at 7MS bin FL 0.60-0.65 by development of wheat-Ae. geniculata structural aberrations. The development of wheat-wild relative chromosomal structure aberrations not only provides novel germplasm resources for wheat improvement, but also aids in mapping desirable genes to specific chromosomal regions. Aegilops geniculata (2n = 4x = 28, UUMM), a wild relative of common wheat, possesses many favorable genes. In this study, Ae. geniculata chromosome 7M was identified as harboring a purple coleoptile gene by phenotypic evaluation of Chinese Spring (CS)-Ae. geniculata addition and substitution lines. To construct a physical map of chromosome 7M and localize the purple coleoptile gene, 59 molecular markers specific to 7M were developed, and 43 wheat-Ae. geniculata 7M chromosome structure aberrations were generated based on chromosome centromeric breakage-fusion and ph1b-induced homoeologous recombination. Segment sizes and breakpoint positions of each 7M structure aberration were further characterized using in situ hybridization and molecular marker analysis. Consequently, a physical map of chromosome 7M was constructed with 59 molecular markers, comprising six bins with 28 markers on 7MS and six bins with 31 markers on 7ML, and the purple coleoptile gene was mapped to an interval of FL 0.60-0.65 on 7MS. The newly developed wheat-Ae. geniculata 7M structural aberrations and the physical map of 7M will facilitate the transfer and utilization of desirable genes from 7M in the future.
构建了节节麦7M染色体的物理图谱，并通过培育小麦-节节麦结构变异体将一个新的紫色胚芽鞘基因定位在7MS区间FL 0.60 - 0.65处。小麦与野生近缘种染色体结构变异体的培育不仅为小麦改良提供了新的种质资源，也有助于将优良基因定位到特定的染色体区域。节节麦（2n = 4x = 28，UUMM）是普通小麦的野生近缘种，拥有许多优良基因。在本研究中，通过对中国春（CS）-节节麦附加系和代换系的表型评价，确定节节麦7M染色体携带一个紫色胚芽鞘基因。为了构建7M染色体的物理图谱并定位紫色胚芽鞘基因，开发了59个7M特异的分子标记，并基于染色体着丝粒断裂-融合和ph1b诱导的同源重组产生了43个小麦-节节麦7M染色体结构变异体。利用原位杂交和分子标记分析进一步鉴定了每个7M结构变异体的片段大小和断点位置。因此，构建了一个包含59个分子标记的7M染色体物理图谱，其中7MS上有6个区间，包含28个标记，7ML上有6个区间，包含31个标记，紫色胚芽鞘基因被定位到7MS上FL 0.60 - 0.65的区间内。新培育的小麦-节节麦7M结构变异体和7M物理图谱将有助于未来7M上优良基因的转移和利用。
