The State Key Laboratory of Wheat and Maize Crop Science, College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, PR China.
College of Agronomy, Nanjing Agricultural University, Nanjing, 210000, PR China.
Nat Commun. 2024 Mar 19;15(1):2449. doi: 10.1038/s41467-024-46814-7.
Wheat powdery mildew is one of the most destructive diseases threatening global wheat production. The wild relatives of wheat constitute rich sources of diversity for powdery mildew resistance. Here, we report the map-based cloning of the powdery mildew resistance gene Pm13 from the wild wheat species Aegilops longissima. Pm13 encodes a mixed lineage kinase domain-like (MLKL) protein that contains an N-terminal-domain of MLKL (MLKL_NTD) domain in its N-terminus and a C-terminal serine/threonine kinase (STK) domain. The resistance function of Pm13 is validated by mutagenesis, gene silencing, transgenic assay, and allelic association analyses. The development of introgression lines with significantly reduced chromosome segments of Ae. longissima encompassing Pm13 enables widespread deployment of this gene into wheat cultivars. The cloning of Pm13 may provide valuable insights into the molecular mechanisms underlying Pm13-mediated powdery mildew resistance and highlight the important roles of kinase fusion proteins (KFPs) in wheat immunity.
小麦白粉病是威胁全球小麦生产的最具破坏性的疾病之一。小麦的野生近缘种是抗白粉病的丰富多样性来源。在这里，我们报告了从野生小麦物种长穗偃麦草中克隆出的白粉病抗性基因 Pm13。Pm13 编码一个混合谱系激酶结构域样（MLKL）蛋白，其 N 端包含一个 MLKL_NTD 结构域，C 端包含一个丝氨酸/苏氨酸激酶（STK）结构域。通过突变、基因沉默、转基因试验和等位基因关联分析验证了 Pm13 的抗性功能。通过与长穗偃麦草的染色体片段进行基因渗入，获得了携带 Pm13 显著减少的染色体片段的导入系，从而使该基因能够广泛应用于小麦品种。Pm13 的克隆为 Pm13 介导的白粉病抗性的分子机制提供了有价值的见解，并强调了激酶融合蛋白（KFPs）在小麦免疫中的重要作用。
