Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu, 611731, Sichuan, China.
Plant Breeding Institute, School of Life and Environmental Sciences, The University of Sydney, Cobbitty, NSW, 2570, Australia.
Planta. 2021 Jan 5;253(1):22. doi: 10.1007/s00425-020-03552-6.
A physical map of Thinopyrum intermedium chromosome 7J was constructed using translocation mapping, and a new seedling purple coleoptile gene was mapped to the bin of FL 0.35-0.63 of 7JS. Thinopyrum intermedium (2n = 6x = 42, JJJJStSt), a wild relative of common wheat, harbors numerous beneficial genes for wheat improvement. Previous studies showed that wheat-Th. intermedium partial amphiploid TAF46 and its derived addition line L1 had a purple coleoptile, which was derived from Th. intermedium chromosome 7J. To identify and physically map the purple coleoptile gene, 12 wheat-Th. intermedium 7J translocation lines were analyzed by sequential multicolor fluorescence in situ hybridization (mc-FISH), PCR-based landmark unique gene (PLUG) and intron targeting (IT) markers. A physical map of the 7J chromosome was constructed, consisting of eight chromosomal bins with 89 markers. Seedling evaluation of the coleoptile colors of all tested materials indicated that the purple coleoptile gene was located to the bin with a fraction length (FL) of 0.35-0.63 on chromosome 7JS. Furthermore, based on the syntenic relationships between Th. intermedium and wheat chromosomes, we developed a new chromosome 7J-specific EST-PCR marker from the chromosomal region corresponding to the purple coleoptile gene through the Triticeae multi-omics database. The approach of designing chromosome-specific markers has facilitated fine mapping of the Thinopyrum-specific purple coleoptile gene, and these translocation lines will be valuable for studying the function of the purple coleoptile gene in anthocyanin biosynthesis.
利用易位作图法构建了中间偃麦草 7J 染色体的物理图谱，并将一个新的幼苗紫鞘基因定位到 7JS 的 FL 0.35-0.63 -bin。中间偃麦草（2n=6x=42，JJJJStSt）是普通小麦的野生近缘种，含有许多有益于小麦改良的有益基因。先前的研究表明，小麦-中间偃麦草部分异源六倍体 TAF46 及其衍生的添加系 L1 具有紫鞘，这源自中间偃麦草 7J 染色体。为了鉴定和物理图谱紫鞘基因，通过顺序多色荧光原位杂交（mc-FISH）、基于 PCR 的地标独特基因（PLUG）和内含子靶向（IT）标记对 12 个小麦-中间偃麦草 7J 易位系进行了分析。构建了 7J 染色体的物理图谱，由 89 个标记组成的 8 个染色体 bin。对所有测试材料的鞘颜色的幼苗评价表明，紫鞘基因位于 7JS 染色体上 FL 为 0.35-0.63 的 bin 中。此外，基于中间偃麦草和小麦染色体之间的同线性关系，我们通过 Triticeae 多组学数据库，从对应于紫鞘基因的染色体区域开发了一个新的中间偃麦草 7J 特异性 EST-PCR 标记。设计染色体特异性标记的方法促进了中间偃麦草特有的紫鞘基因的精细图谱定位，这些易位系将有助于研究紫鞘基因在花青素生物合成中的功能。
