Yuk Kyung-Jin, Kim You-Tae, Huh Chul-Sung, Lee Ju-Hoon
Department of Food Science and Biotechnology, Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea.
Graduate School of International Agricultural Technology, Institute of Green-Bio Science and Technology, Seoul National University, Pyeongchang 25354, Republic of Korea.
Int J Syst Evol Microbiol. 2018 May;68(5):1725-1731. doi: 10.1099/ijsem.0.002737. Epub 2018 Mar 27.
Strain PFL01 was isolated from traditional Korean fermented clam, jogae-jeotgal, and characterized. The strain was a facultative anaerobic, Gram-stain-negative bacterium that was rod-shaped, motile and beige-pigmented. The phylogenetic sequence analysis based on the 16S rRNA gene from PFL01 revealed that it was closely related to Lelliottia nimipressuralis LMG 10245 and Lelliottia amnigena LMG 2784 with 99.3 and 99.3 % sequence identities, respectively. Multilocus sequence type analysis of concatenated partial aptD, gyrB, infB and rpoB gene sequences showed a clear distinction of strain PFL01 from its closest related type strains. The discrimination was also supported by unique repetitive extragenic palindromic PCR (Rep-PCR, ERIC-PCR) fingerprint patterns. In addition, results from average nucleotide identity analyses with other species were less than 85 %. vitek and API analyses revealed distinct characteristics from other species of Lelliottia. The cellular fatty acid profile of the strain consisted of C16 : 0, cyclo-C17 : 0, C16 : 1ω7c/C16 : 1ω6c and C18 : 1ω7c/C18 : 1ω6c as major components. The whole genome of PFL01 was 4.6 Mb with a G+C content of 55.3 mol%. Based on these results, strain PFL01 was classified as a novel species of the genus Lelliottia, for which the name Lelliottia jeotgali sp. nov. is proposed. The type strain in PFL01 (=KCCM 43247=JCM 31901).
从韩国传统发酵蛤蜊（jogae-jeotgal）中分离出菌株PFL01并对其进行了鉴定。该菌株为兼性厌氧菌，革兰氏染色阴性，呈杆状，具运动性，有米色色素沉着。基于PFL01的16S rRNA基因进行的系统发育序列分析表明，它与压力利氏菌（Lelliottia nimipressuralis）LMG 10245和嗜水利氏菌（Lelliottia amnigena）LMG 2784密切相关，序列同一性分别为99.3%和99.3%。对串联的部分aptD、gyrB、infB和rpoB基因序列进行多位点序列分型分析，结果显示菌株PFL01与其最密切相关的模式菌株明显不同。这种区分也得到了独特的重复外显子回文PCR（Rep-PCR，ERIC-PCR）指纹图谱的支持。此外，与其他物种的平均核苷酸同一性分析结果低于85%。Vitek和API分析显示其具有与利氏菌属其他物种不同的特征。该菌株的细胞脂肪酸谱主要由C16 : 0、环C17 : 0、C16 : 1ω7c/C16 : 1ω6c和C18 : 1ω7c/C18 : 1ω6c组成。PFL01的全基因组为4.6 Mb，G+C含量为55.3 mol%。基于这些结果，菌株PFL01被归类为利氏菌属的一个新物种，提议将其命名为嗜蛤利氏菌（Lelliottia jeotgali sp. nov.）。模式菌株为PFL01（=KCCM 43247=JCM 31901）。
