Ma Kaixuan, Yang Lei, Li Wenzhao, Chen Kai, Shang Luoran, Bai Yushu, Zhao Yuanjin
Department of Orthopedics, Shanghai Changhai Hospital, Naval Medical University, Shanghai, 200433, China.
Oujiang Laboratory (Zhejiang Lab for Regenerative Medicine, Vision and Brain Health), Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, 325001, China.
Mater Today Bio. 2024 Nov 23;29:101363. doi: 10.1016/j.mtbio.2024.101363. eCollection 2024 Dec.
Hydrogel microspheres hold great promise as scaffolds for bone repair. Their hydrated matrix, biocompatibility, and functional properties make them an attractive choice in regenerative medicine. However, the irregularity of defect requires shape adaptability of the microspheres. Additionally, there is still room for improvement regarding the component of the microspheres to achieve sufficient bioactivity. Here, we prepare multi-bioactive microspheres composed of methacrylated silk fibroin (SFMA) microfluidic electrospray. Magnesium ascorbyl phosphate (MAP) is encapsulated within the microspheres, whose sustained release facilitates angiogenesis and osteogenic differentiation. The microspheres are further coated with a polydopamine (PDA) layer, allowing them to assemble into a scaffold that conforms to the non-uniform contours of bone defects. The photothermal conversion capability of PDA also provides mild photothermal stimulation to further promote bone regeneration. Based on the synergistic effects, our experiments demonstrated that the microsphere scaffold effectively promotes bone defect healing. Thus, this multi-bioactive scaffold offers a versatile strategy for bone repair with promising clinical potential.
水凝胶微球作为骨修复支架具有巨大潜力。其水合基质、生物相容性和功能特性使其成为再生医学中颇具吸引力的选择。然而，缺损的不规则性要求微球具有形状适应性。此外，在微球的成分方面仍有改进空间，以实现足够的生物活性。在此，我们通过微流控电喷雾制备了由甲基丙烯酸化丝素蛋白（SFMA）组成的多生物活性微球。抗坏血酸磷酸镁（MAP）被包裹在微球内，其持续释放促进血管生成和成骨分化。微球进一步涂覆有聚多巴胺（PDA）层，使其能够组装成符合骨缺损不均匀轮廓的支架。PDA的光热转换能力还提供温和的光热刺激，以进一步促进骨再生。基于协同效应，我们的实验表明微球支架能有效促进骨缺损愈合。因此，这种多生物活性支架为骨修复提供了一种具有广阔临床潜力的通用策略。
