Yang Lite, Fang Lisa Z, Lynch Michelle R, Xu Chang S, Hahm Hannah J, Zhang Yufen, Heitmeier Monique R, Costa Vincent D, Samineni Vijay K, Creed Meaghan C
Department of Anesthesiology, Washington University Pain Center, Washington University School of Medicine, St. Louis, MO, USA.
Neuroscience Graduate Program, Division of Biology & Biomedical Sciences, Washington University School of Medicine, St. Louis, MO, USA.
Sci Adv. 2024 Dec 13;10(50):eadq6017. doi: 10.1126/sciadv.adq6017. Epub 2024 Dec 11.
The ventral pallidum (VP) is critical for motivated behaviors. While contemporary work has begun to elucidate the functional diversity of VP neurons, the molecular heterogeneity underlying this functional diversity remains incompletely understood. We used single-nucleus RNA sequencing and in situ hybridization to define the transcriptional taxonomy of VP cell types in mice, macaques, and baboons. We found transcriptional conservation between all three species, within the broader neurochemical cell types. Unique dopaminoceptive and cholinergic subclusters were identified and conserved across both primate species but had no homolog in mice. This harmonized consensus VP cellular atlas will pave the way for understanding the structure and function of the VP and identified key neuropeptides, neurotransmitters, and neurotransmitter receptors that could be targeted within specific VP cell types for functional investigations.
腹侧苍白球（VP）对动机行为至关重要。尽管当代研究已开始阐明VP神经元的功能多样性，但其功能多样性背后的分子异质性仍未完全被理解。我们使用单核RNA测序和原位杂交来定义小鼠、猕猴和狒狒中VP细胞类型的转录分类。我们发现在更广泛的神经化学细胞类型中，所有这三个物种之间存在转录保守性。独特的多巴胺感受性和胆碱能亚群在两种灵长类物种中均被鉴定出来并具有保守性，但在小鼠中没有同源物。这个统一的腹侧苍白球细胞图谱将为理解腹侧苍白球的结构和功能铺平道路，并确定了关键神经肽、神经递质和神经递质受体，这些可以在特定的腹侧苍白球细胞类型中作为功能研究的靶点。
