Ferrari Carolina Ruis, Kitamoto Karolyne Sayuri de Araujo, Pelá Vinicius Taioqui, Taira Éven Akemi, Araújo Tamara Teodoro, Thomassian Larissa Tercilia Grizzo, Henrique-Silva Flávio, Pessan Juliano Pelim, Buzalaf Marília Afonso Rabelo
Universidade de São Paulo - USP, Bauru School of Dentistry, Department of Biological Sciences, Bauru, SP, Brazil.
Universidade Federal de São Carlos - UFSCar, Department of Genetics and Evolution, São Carlos, SP, Brazil.
Braz Oral Res. 2024 Dec 9;38:e124. doi: 10.1590/1807-3107bor-2024.vol38.0124. eCollection 2024.
The objective of this in vitro study was to assess the efficacy of CaneCPI-5, either alone or in combination with various concentrations of sodium trimetaphosphate (TMP) in protecting against initial enamel erosion. A total of 135 bovine enamel specimens were prepared and categorized into nine groups (n/group=15) according to the following treatments: Deionized water; Commercial solution (Elmex Erosion ProtectionTM); 0.1 mg/mL CaneCPI-5; 0.5% TMP; 1.0% TMP; 3.0% TMP; 0.1 mg/mL CaneCPI-5+0.5% TMP; 0.1 mg/mL CaneCPI-5+1.0%TMP; and 0.1 mg/mL CaneCPI-5+3.0%TMP. The specimens were treated with the respective solutions for 2 h, followed by acquired enamel pellicle formation for 2 h and exposure to 0.65% citric acid (CA) for 1 min. These procedures were repeated once a day for three consecutive days. Demineralization was assessed by the percentage change in surface hardness (%CSH) and calcium release into CA, analyzed by the Arsenazo III method. The data were evaluated using Kruskal-Wallis/Dunn's tests. Regarding %CSH, CaneCPI-5+3.0%TMP was the most effective treatment when compared to the CaneCPI-5 group alone. As for calcium release into CA, the CaneCPI-5+0.5% TMP and CaneCPI-5 groups (both with lower calcium release) did not significantly differ from the commercial solution. In conclusion, combination of CaneCPI-5 with TMP enhances the protective potential against initial enamel erosion in vitro.
本体外研究的目的是评估CaneCPI-5单独使用或与不同浓度的三聚磷酸钠（TMP）联合使用时，对预防早期牙釉质侵蚀的效果。共制备了135个牛牙釉质标本，并根据以下处理方式分为9组（每组n = 15）：去离子水；市售溶液（Elmex Erosion ProtectionTM）；0.1 mg/mL CaneCPI-5；0.5% TMP；1.0% TMP；3.0% TMP；0.1 mg/mL CaneCPI-5 + 0.5% TMP；0.1 mg/mL CaneCPI-5 + 1.0% TMP；以及0.1 mg/mL CaneCPI-5 + 3.0% TMP。将标本用相应溶液处理2小时，随后形成牙釉质薄膜2小时，再暴露于0.65%柠檬酸（CA）中1分钟。这些步骤连续三天每天重复一次。通过表面硬度百分比变化（%CSH）评估脱矿情况，并采用偶氮胂III法分析钙释放到CA中的情况。数据使用Kruskal-Wallis/Dunn检验进行评估。关于%CSH，与单独的CaneCPI-5组相比，CaneCPI-5 + 3.0% TMP是最有效的处理方式。至于钙释放到CA中的情况，CaneCPI-5 + 0.5% TMP组和CaneCPI-5组（两者钙释放量较低）与市售溶液相比无显著差异。总之，CaneCPI-5与TMP联合使用可增强体外对早期牙釉质侵蚀的防护潜力。
