Eskalen Alper, Işık Eray, Ozdemir Ilkay, Keskin Ilknur, Keskin Mustafa, Karacaoglan Naci
Department of Plastic, Reconstructive and Aesthetic Surgery Department, Necip Fazil City Hospital, Karacasu Kirim Gaziantep road 12. Km, Dulkadiroglu,, Kahramanmaras, Turkey.
Department of Plastic, Reconstructive and Aesthetic surgery, Medipol University School of Medicine, TEM avrupa otoyolu Goztepe cikisi 1, 34214, Bagcilar,, Istanbul, Turkey.
Aesthetic Plast Surg. 2025 Mar;49(5):1387-1395. doi: 10.1007/s00266-024-04580-3. Epub 2024 Dec 11.
Fat graft applications are one of the most frequently used procedures in reconstructive and aesthetic surgery. Fat graft survival rate is variable and depends on many parameters. Those are the features of recipient area, method of obtaining the fat graft, the procedures applied to the harvest to get pure fat graft and the application techniques. In this study, we aimed to find the optimal revolutions per minute(rpm) and duration to be used in the centrifugation technique, which is one of the most frequently used methods in fat grafting. In our study, we used perilipin protein as a survival marker. Liposuction samples were obtained from 10 Caucasian women individuals; whose age range is between 22 and 29 years without any other reported health problem. Fat grafts from all patients were taken by the same surgical team by the same liposuction procedure. The grafts obtained in our study were divided into 6 different groups, these are A1 group (1 minute-1000 rpm) A2 group (3 minutes-1000 rpm) A3 group (5 minutes-1000 rpm) B1 group (1 minute-3000 rpm) B2 group (3 minutes-3000 rpm) and B3 group (5 minutes-3000 rpm). Perilipin expression measurements were made in the sections taken by immunohistochemistry technique. Three minutes-3000 rpm combination (B2 group) was found to be perilipin stained statistically significantly higher than other combinations. This study seeks to make a notable contribution to the literature by demonstrating the relation between frequently used centrifuge combinations and measuring the expression of perilipin. This protein's role in adipocyte regulation is becoming increasingly apparent.Level of Evidence III This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
脂肪移植应用是整形重建外科和美容外科中最常用的手术之一。脂肪移植成活率各不相同，取决于许多因素。这些因素包括受区特征、获取脂肪移植体的方法、为获得纯净脂肪移植体而在采集过程中采用的操作以及应用技术。在本研究中，我们旨在找出脂肪移植中最常用方法之一的离心技术所使用的最佳每分钟转速（rpm）和时长。在我们的研究中，我们将围脂滴蛋白用作存活标志物。抽脂样本取自10名白种女性个体；年龄在22至29岁之间，无其他健康问题报告。所有患者的脂肪移植体均由同一手术团队通过相同的抽脂手术获取。我们研究中获得的移植体被分为6个不同组，即A1组（1分钟 - 1000 rpm）、A2组（3分钟 - 1000 rpm）、A3组（5分钟 - 1000 rpm）、B1组（1分钟 - 3000 rpm）、B2组（3分钟 - 3000 rpm）和B3组（5分钟 - 3000 rpm）。采用免疫组织化学技术在切片上进行围脂滴蛋白表达测量。发现3分钟 - 3000 rpm组合（B2组）的围脂滴蛋白染色在统计学上显著高于其他组合。本研究旨在通过证明常用离心组合与测量围脂滴蛋白表达之间的关系，为文献做出显著贡献。这种蛋白质在脂肪细胞调节中的作用日益明显。证据等级III 本刊要求作者为每篇文章指定证据等级。有关这些循证医学评级的完整描述，请参阅目录或作者在线指南www.springer.com/00266 。
