Salinas Harry M, Broelsch G Felix, Fernandes Justin R, McCormack Michael C, Meppelink Amanda M, Randolph Mark A, Colwell Amy S, Austen William G
Boston, Mass. From the Division of Plastic and Reconstructive Surgery, Massachusetts General Hospital.
Plast Reconstr Surg. 2014 Oct;134(4):675-683. doi: 10.1097/PRS.0000000000000524.
Centrifugation is a popular processing method, with an unclear mechanism of action. Hypotheses include fat concentration, reduced inflammatory response by removal of blood, and concentration of adipose-derived stem cells. The authors performed multiple experiments to determine the role of centrifugation and compared it with a different processing method (mesh/gauze technique).
Lipoaspirate components were quantified after centrifugation at increasing speed to determine concentration efficacy. For comparison, the authors quantified the concentration efficacy of mesh/gauze. They also compared the number of adipose-derived stem cells isolated by either method. To determine the effects of each component, they compared fat alone to fat mixed with various spinoff components in a mouse model. They also compared centrifugation to mesh/gauze.
The adipocyte fraction remains constant above 5000 g, whereas 1200 g results in 91 percent concentrated fat. Mesh/gauze also results in 90 percent concentrated fat. The number of adipose-derived stem cells in 1 g of fat was 1603 ± 2020 and 1857 ± 1832 in the centrifuge and mesh/gauze groups, respectively (p = 0.86). Five "add-back" groups were created: fat plus oil, fat plus surgical tumescence, fat plus fresh tumescence, fat plus cell pellets and fresh tumescence, and fat plus cell pellets. The fat-only group had better retention than the groups mixed with tumescence, regardless of whether it was surgical, fresh, or had cell pellets. Oil did not affect grafts. Centrifugation at 1200 g was equivalent to mesh/gauze (0.73 ± 0.12 g and 0.72 ± 0.13 g, respectively).
Centrifugation improves graft retention by concentration of the adipocyte fraction. The concentration efficacy of mesh/gauze is equivalent to centrifugation at 1200 g, with equivalent in vivo outcomes.
离心是一种常用的处理方法，但其作用机制尚不清楚。假说是脂肪浓缩、通过去除血液减少炎症反应以及脂肪来源干细胞的浓缩。作者进行了多项实验以确定离心的作用，并将其与另一种处理方法（滤网/纱布技术）进行比较。
以递增速度离心后对脂肪抽吸物成分进行定量，以确定浓缩效果。为作比较，作者对滤网/纱布的浓缩效果进行了定量。他们还比较了通过两种方法分离的脂肪来源干细胞的数量。为确定各成分的作用，他们在小鼠模型中将单纯脂肪与混合了各种副产品成分的脂肪进行比较。他们还将离心与滤网/纱布进行比较。
脂肪细胞部分在5000 g以上保持恒定，而1200 g可使91%的脂肪得到浓缩。滤网/纱布也可使90%的脂肪得到浓缩。离心组和滤网/纱布组每1 g脂肪中脂肪来源干细胞的数量分别为1603±2020和1857±1832（p = 0.86）。创建了五个“回加”组：脂肪加油、脂肪加手术肿胀液、脂肪加新鲜肿胀液、脂肪加细胞沉淀和新鲜肿胀液以及脂肪加细胞沉淀。无论是否为手术用、新鲜的或含有细胞沉淀的肿胀液，单纯脂肪组的留存率均优于与肿胀液混合的组。油对移植物无影响。1200 g离心与滤网/纱布效果相当（分别为0.73±0.12 g和0.72±0.13 g）。
离心通过浓缩脂肪细胞部分提高移植物留存率。滤网/纱布的浓缩效果与1200 g离心相当，体内结果也相当。
