Zhao Qian, Wang Ruoyi, Song Yan, Lu Juan, Zhou Bingjie, Song Fang, Zhang Lijuan, Huang Qianqian, Gong Jing, Lei Jingjing, Dong Suomeng, Gu Qin, Borriss Rainer, Gao Xuewen, Wu Huijun
Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.
Key Laboratory of Soybean Disease and Pest Control (Ministry of Agriculture and Rural Affairs), Nanjing Agricultural University, Nanjing, China.
NPJ Biofilms Microbiomes. 2024 Dec 11;10(1):145. doi: 10.1038/s41522-024-00627-0.
Plant-beneficial Pseudomonas and Bacillus have been extensively studied and applied in biocontrol of plant diseases. However, there is less known about their interaction within two-strain synthetic communities (SynCom). Our study revealed that Pseudomonas protegens Pf-5 inhibits the growth of several Bacillus species, including Bacillus velezensis. We established a two-strain combination of Pf-5 and DMW1 to elucidate the interaction. In this combination, pyoluteorin conferred the competitive advantage of Pf-5. Noteworthy, pyoluteorin-deficient Pf-5 cooperated with DMW1 in biofilm formation, production of metabolites, root colonization, tomato bacterial wilt disease control, as well as in cooperation with beneficial bacteria in tomato rhizosphere, such as Bacillus spp. RNA-seq analysis and RT-qPCR also proved the pyoluteorin-deficient Pf-5 mutant improved cell motility and metabolite production. This study suggests that the cooperative effect of Bacillus-Pseudomonas consortia depends on the balance of pyoluteorin. Our finding needs to be considered in developing efficient SynCom in sustainable agriculture.
对植物有益的假单胞菌属和芽孢杆菌属已得到广泛研究，并应用于植物病害的生物防治。然而，关于它们在双菌株合成群落（SynCom）中的相互作用，人们了解较少。我们的研究表明，荧光假单胞菌Pf-5会抑制包括贝莱斯芽孢杆菌在内的几种芽孢杆菌属细菌的生长。我们构建了Pf-5和DMW1的双菌株组合来阐明它们之间的相互作用。在这种组合中，绿脓菌素赋予了Pf-5竞争优势。值得注意的是，缺乏绿脓菌素的Pf-5与DMW1在生物膜形成、代谢产物产生、根部定殖、番茄青枯病防治以及与番茄根际有益细菌（如芽孢杆菌属）的合作方面表现出协同作用。RNA测序分析和实时定量聚合酶链反应也证明，缺乏绿脓菌素的Pf-5突变体提高了细胞运动性和代谢产物产量。这项研究表明，芽孢杆菌-假单胞菌联合体的协同效应取决于绿脓菌素的平衡。在可持续农业中开发高效的合成群落时，需要考虑我们的这一发现。
