Zhang Meiyu, Wu Shanyou, Chen Peng, Shao Lin, Shen Zizhu, Zhao Yinjuan
Collaborative Innovation Center of Sustainable Forestry in Southern China, Nanjing 210037, China.
College of Forestry, Nanjing Forestry University, Nanjing 210037, China.
Microorganisms. 2025 Apr 13;13(4):896. doi: 10.3390/microorganisms13040896.
Cyclic diguanosine monophosphate (c-di-GMP) is a second messenger that plays a crucial role in regulating biofilm development, yet the role in Gram-positive bacteria remains elusive. Here, we demonstrated that dispersed cells from biofilms of FZB42 exhibit a unique phenotype and gene expression compared to planktonic cells. Transcriptomic analysis revealed 1327 downregulated and 1298 upregulated genes, among which the c-di-GMP phosphodiesterase coding gene was remarkably upregulated. Deletion of the gene led to elevated c-di-GMP levels accompanied by reduced amounts of "actively dispersed cells" from the pellicle and the capacity of motility. Deletion of , , and resulted in increased c-di-GMP levels and reduced biofilm dispersal ability. Also, the level of c-di-GMP was increased when adding the cues of inhibition biofilm dispersal such as glucose and calcium ions. Collectively, these present findings suggest the c-di-GMP level is negatively correlated with biofilm dispersal in FZB42, which sheds new light on biofilm regulation in FZB42.
环二鸟苷单磷酸（c-di-GMP）是一种第二信使，在调节生物膜形成过程中发挥关键作用，但其在革兰氏阳性菌中的作用仍不清楚。在此，我们证明，与浮游细胞相比，来自FZB42生物膜的分散细胞表现出独特的表型和基因表达。转录组分析显示1327个基因下调，1298个基因上调，其中编码c-di-GMP磷酸二酯酶的基因显著上调。该基因的缺失导致c-di-GMP水平升高，同时来自菌膜的“主动分散细胞”数量减少以及运动能力下降。基因、和的缺失导致c-di-GMP水平升高以及生物膜分散能力降低。此外，添加抑制生物膜分散的信号分子如葡萄糖和钙离子时，c-di-GMP水平升高。总的来说，这些研究结果表明FZB42中c-di-GMP水平与生物膜分散呈负相关，这为FZB42中生物膜调控提供了新的线索。
