Lin Weifeng, Yang Yifan, Zhu Yurong, Pan Rong, Liu Chaonan, Pan Jiyang
Department of Neurology, The Tenth Affiliated Hospital, Southern Medical University (Dongguan People's Hospital), Dongguan, Guangdong, 523000, People's Republic of China.
Department of Psychiatry, Sleep Medicine Center, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong, 510632, People's Republic of China.
Nat Sci Sleep. 2024 Dec 7;16:1959-1972. doi: 10.2147/NSS.S472675. eCollection 2024.
Despite recent findings suggesting an altered gut microbiota in those suffering from insomnia disorder (ID), research into the gut microbiota, oral microbiota, serum metabolites, and their interactions in patients with ID is sparse.
We collected a total of 114 fecal samples, 133 oral cavity samples and 20 serum samples to characterize the gut microbiota, oral microbiota and serum metabolites in a cohort of 76 ID patients (IDs) and 59 well-matched healthy controls (HCs). We assessed the microbiota as potentially biomarkers for ID for ID by 16S rDNA sequencing and elucidated the interactions involving gut microbiota, oral microbiota and serum metabolites in ID in conjunction with untargeted metabolomics.
Gut and oral microbiota of IDs were dysbiotic. Gut and oral microbial biomarkers could be used to differentiate IDs from HCs. Eleven significantly altered serum metabolites, including adenosine, phenol, and phenol sulfate, differed significantly between groups. In multi-omics analyses, adenosine showed a positive correlation with genus_ (0.029) and total sleep time (=0.016). Additionally, phenol and phenol sulphate had a negative correlation with genus (=0.0059; =0.0059) and a positive correlation with Pittsburgh Sleep Quality Index (=0.006; =0.006) and Insomnia Severity Index (=0.021; =0.021).
Microbiota and serum metabolite changes in IDs are strongly correlated with clinical parameters, implying mechanistic links between altered bacteria, serum metabolites and ID. This study offers novel perspective into the interaction among gut microbiota, oral microbiota, and serum metabolites for ID.
尽管最近的研究结果表明失眠症（ID）患者的肠道微生物群发生了改变，但对ID患者的肠道微生物群、口腔微生物群、血清代谢物及其相互作用的研究却很少。
我们总共收集了114份粪便样本、133份口腔样本和20份血清样本，以表征76名ID患者（IDs）和59名匹配良好的健康对照者（HCs）的肠道微生物群、口腔微生物群和血清代谢物。我们通过16S rDNA测序评估微生物群作为ID的潜在生物标志物，并结合非靶向代谢组学阐明ID中涉及肠道微生物群、口腔微生物群和血清代谢物的相互作用。
IDs的肠道和口腔微生物群失调。肠道和口腔微生物生物标志物可用于区分IDs和HCs。11种显著改变的血清代谢物，包括腺苷、苯酚和苯酚硫酸盐，在两组之间有显著差异。在多组学分析中，腺苷与属（0.029）和总睡眠时间（=0.016）呈正相关。此外，苯酚和苯酚硫酸盐与属（=0.0059；=0.0059）呈负相关，与匹兹堡睡眠质量指数（=0.006；=0.006）和失眠严重程度指数（=0.021；=0.021）呈正相关。
IDs中的微生物群和血清代谢物变化与临床参数密切相关，这意味着细菌改变、血清代谢物与ID之间存在机制联系。本研究为ID患者的肠道微生物群、口腔微生物群和血清代谢物之间的相互作用提供了新的视角。
