从西瓜壳针孢中分离出的一种新型威克病毒的分子特征
Molecular characterization of a novel victorivirus isolated from Stagonosporopsis citrulli.
作者信息
Ma Xiaofang, Zhang Xing, Zhai Lifeng, Jiang Yingchun, Moffett Peter, Zhang Yu, Song Fang, Song Xin, Wang Zhijing, He Ligang, Ji Shengmei, Wu Liming
机构信息
Hubei Key Laboratory of Germplasm Innovation and Utilization of Fruit trees, Research Institute of Fruit and Tea, Hubei Academy of Agricultural Sciences, Wuhan, Hubei, 430064, P. R. China.
College of Life Science and Technology, Yangtze Normal University, Chongqing, 408000, China.
出版信息
Arch Virol. 2024 Dec 12;170(1):14. doi: 10.1007/s00705-024-06185-2.
Members of the fungal genus Stagonosporopsis are important plant pathogens that can cause severe disease on a wide range of economically important plants and crops. Here, a novel victorivirus, tentatively named "Stagonosporopsis citrulli victorivirus 1" (SciVV1), was isolated from S. citrulli isolate HS2-8. The SciVV1 genome is 5,163 nucleotides in length, with a predicted GC content of 63.85%, and contains two open reading frames (ORF1 and ORF2), which overlap at an AUGA sequence. ORF1 and ORF2 are predicted to encode a coat protein (CP) and an RNA-dependent RNA polymerase (RdRp). A phylogenetic tree based on RdRp amino acid (aa) sequences showed that SciVV1 clustered together with members of the genus Victorivirus in the family Pseudototiviridae. Our results indicate that SciVV1 is a novel victorivirus. This is the first report of the complete genome sequence of SciVV1 infecting S. citrulli.
葡萄座腔菌属的成员是重要的植物病原体，可在多种经济上重要的植物和作物上引发严重病害。在此，从西瓜葡萄座腔菌分离株HS2-8中分离出一种新型的威克病毒，暂命名为“西瓜葡萄座腔菌威克病毒1”（SciVV1）。SciVV1基因组长度为5163个核苷酸，预测的GC含量为63.85%，包含两个开放阅读框（ORF1和ORF2），它们在AUGA序列处重叠。预测ORF1和ORF2分别编码一种衣壳蛋白（CP）和一种RNA依赖的RNA聚合酶（RdRp）。基于RdRp氨基酸（aa）序列构建的系统发育树表明，SciVV1与假总病毒科威克病毒属的成员聚集在一起。我们的结果表明，SciVV1是一种新型的威克病毒。这是关于感染西瓜葡萄座腔菌的SciVV1完整基因组序列的首次报道。
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