A self-fixing xanthan gum hydrogel membrane with ROS scavenging capability for the prevention of postoperative abdominal adhesion.

Postoperative abdominal adhesions are a common complication of surgery and are caused by inflammation, tissue damage, and hypoxia. To address this issue, we prepared a SC-Xg hydrogel membrane by crosslinking xanthan gum (Xg) and sodium citrate (SC) through a dehydration condensation reaction with a crosslinking density of 39.4 %. Moreover, the mechanical performance of the SC-Xg hydrogel membrane could be enhanced by adjusting the concentration of SC. The 1.2-SC-Xg hydrogel membrane exhibited the highest stress resistance. The SC-Xg hydrogel membrane retains the excellent self-fixing ability, biocompatibility, and biodegradability of Xg while incorporating the antioxidant properties of SC. In vitro and in vivo experiments confirmed that the SC-Xg hydrogel membrane can completely cover irregular surgical sites and act as the most effective barrier against adhesions, fully exerting both physical and biological effects. Furthermore, molecular mechanism studies revealed that the hydrogel membrane primarily activated the Nrf2 signaling pathway in a concentration-dependent manner, enabling the SC-Xg hydrogel membrane to suppress postoperative oxidative stress reactions (ROS), reduce inflammation levels of IL-6 and TNF-α, reduce fibrosis, and effectively prevent the formation of abdominal adhesions. In conclusion, the SC-Xg hydrogel membrane represents a promising strategy for preventing postoperative abdominal adhesions.