Krishnan Mena Asha, Alimi Olawale A, Kuss Mitchell, Razabdouski Tiffany N, Eksioglu Erika A, Duan Bin, Liu Bo
Mary and Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE, 68198, USA.
Division of Cardiovascular Medicine, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, NE, 68198, USA.
Adv Healthc Mater. 2025 Apr;14(11):e2405238. doi: 10.1002/adhm.202405238. Epub 2025 Mar 6.
Postoperative abdominal adhesions are a common and painful complication after surgery, leading to high healthcare costs and diminished quality of life. This report presents a novel bilayer hydrogel barrier featuring an inner adhesive layer and an outer antiadhesive layer. The inner adhesive layer hydrogel (PT) is prepared by mixing polyethyleneimine (PEI) and thioctic acid (TA). The outer layer (HP) hydrogel is fabricated by the conjugation reaction of thermoresponsive zwitterionic hyaluronic acid, phenylboronic acid, and epigallocatechin gallate complex and polyvinyl alcohol based on dynamic boronic ester bond. The PEI/TA layer enhances attachment to moist tissue surfaces in vivo, and the anti-adhesive layer HP hydrogel promotes biocompatibility and anti-inflammation while minimizing protein adsorption and improving mechanical stability. The bilayer hydrogel (HPPT) exhibited rapid gelation, robust adhesion in dynamic and moist environments, superior viscoelastic properties and cellular biocompatibility. A mouse-cecum abdominal wall adhesion model is utilized to evaluate efficacy, and the HPPT hydrogel shows local retention, anti-inflammatory effect, and inhibits fibrin deposition while minimizing adhesion formation. These findings highlight the innovative structural and functional properties of the HPPT hydrogel, positioning it as a promising therapeutic barrier in peritoneal surgery aimed at reducing postoperative adhesions and enhancing surgical outcomes.
术后腹腔粘连是手术后常见且痛苦的并发症，会导致高昂的医疗费用和生活质量下降。本报告介绍了一种新型双层水凝胶屏障，其具有内层粘附层和外层抗粘附层。内层粘附层水凝胶（PT）通过混合聚乙烯亚胺（PEI）和硫辛酸（TA）制备而成。外层（HP）水凝胶是基于动态硼酸酯键，由热响应性两性离子透明质酸、苯基硼酸和表没食子儿茶素没食子酸酯复合物与聚乙烯醇发生共轭反应制成。PEI/TA层增强了在体内对湿润组织表面的附着，抗粘附层HP水凝胶促进了生物相容性和抗炎作用，同时使蛋白质吸附最小化并提高了机械稳定性。双层水凝胶（HPPT）表现出快速凝胶化、在动态和湿润环境中的强大粘附力、优异的粘弹性和细胞生物相容性。利用小鼠盲肠腹壁粘连模型评估疗效，HPPT水凝胶显示出局部滞留、抗炎作用，并抑制纤维蛋白沉积，同时使粘连形成最小化。这些发现突出了HPPT水凝胶创新的结构和功能特性，使其成为腹膜手术中一种有前景的治疗屏障，旨在减少术后粘连并提高手术效果。
