Fang Rui, Yu Ning, Chen Simeng, Xu Xi, Zhang Jianfa
Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing, 210094, China.
Key Laboratory of Metabolic Engineering and Biosynthesis Technology, Ministry of Industry and Information Technology, Nanjing, 210094, China.
Mater Today Bio. 2025 Jun 24;33:102021. doi: 10.1016/j.mtbio.2025.102021. eCollection 2025 Aug.
Postoperative anti-adhesion materials face significant challenges, including dislocation, the induction of nonspecific tissue adhesions, and secondary fibrinolytic disorders. In this study, we developed a self-leveling, transient, unilaterally bonded Janus patch (J-HD) based on hemoadhican (HD). This patch consists of three distinct functional layers: an anti-adhesion layer, a reinforcement layer, and a wet tissue adhesion layer. Each layer serves a specific purpose: the bottom anti-adhesion layer, composed of HD and Pluronic F127 (PF127), effectively prevents cells, proteins, and tissues from adhering; the middle polyvinyl alcohol (PVA) reinforcement layer enhances the mechanical properties of the film, exhibiting a tensile strength of approximately 2.02 MPa; and the top HD adhesion layer provides strong adhesion to wet tissue with an interfacial toughness of approximately 223.85 J/m. The J-HD patches demonstrate excellent self-leveling ability, unilateral adhesion properties, and effective resistance to fibrin and cell adhesion . In a rat cecum-abdominal wall adhesion model, the J-HD patch exhibited a significant reduction in abdominal adhesions compared to commercially available Interceed® films. Mechanistically, the J-HD patch effectively regulates the fibrinolytic balance by modulating the levels of tissue plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1), promoting a nonadherent state, reducing excessive inflammatory responses, and facilitating the repair of intestinal wall integrity. implantation of the J-HD patch does not exhibit significant acute or chronic toxicity, elicits a favorable host response, and demonstrates biocompatibility. This study presents a potential strategy for the clinical design of Janus patches with anti-migratory and anti-adhesive properties.
术后抗粘连材料面临重大挑战，包括移位、非特异性组织粘连的诱导以及继发性纤维蛋白溶解障碍。在本研究中，我们基于血粘连蛋白（HD）开发了一种自平整、瞬态、单侧粘结的Janus贴片（J-HD）。该贴片由三个不同的功能层组成：抗粘连层、增强层和湿组织粘连层。每层都有特定的作用：底部抗粘连层由HD和普朗尼克F127（PF127）组成，可有效防止细胞、蛋白质和组织粘连；中间的聚乙烯醇（PVA）增强层增强了薄膜的机械性能，拉伸强度约为2.02MPa；顶部的HD粘连层对湿组织具有强粘附力，界面韧性约为223.85J/m。J-HD贴片具有出色的自平整能力、单侧粘附特性以及对纤维蛋白和细胞粘附的有效抗性。在大鼠盲肠-腹壁粘连模型中，与市售的Interceed®薄膜相比，J-HD贴片的腹部粘连明显减少。从机制上讲，J-HD贴片通过调节组织纤溶酶原激活物（t-PA）和纤溶酶原激活物抑制剂-1（PAI-1）的水平，有效调节纤维蛋白溶解平衡，促进非粘附状态，减少过度的炎症反应，并促进肠壁完整性的修复。J-HD贴片植入后未表现出明显的急性或慢性毒性，引发了良好的宿主反应，并证明了生物相容性。本研究为具有抗迁移和抗粘连特性的Janus贴片的临床设计提供了一种潜在策略。
