Fiore Tito, De Santi Nicola, Pietrella Donatella, Barcaccia Mariella, Palmieri Melissa Ida, Lupidi Marco, Mariotti Cesare, Cagini Carlo
Department of Medicine Surgical, Section of Ophthalmology, University of Perugia, S. Maria Della Misericordia Hospital, 06156, Perugia, Italy.
Department of Medicine and Surgery, Medical Microbiology Section, University of Perugia, 06156, Perugia, Italy.
Int Ophthalmol. 2024 Dec 12;45(1):8. doi: 10.1007/s10792-024-03376-3.
To evaluate and compare the mycobacterial load using a mobile laminar airflow (LAF) device in an IVI-dedicated outpatient clean room (OCR) without ventilation systems and in a hospital-based operating theatre (HOT).
This case-control study was conducted in 2 different settings: OCR and HOT during a series of intravitreal injections (IVIs). The Air Microbial analysis was performed using a Surface Air System instrument at three different moments during the IVI sessions in both settings: at the operative site (OS), four meters from the OS (DOS) and in the disinfection room (DR).
At the OS in the OCR, the microbial load was 20.33 ± 16.51 CFU/m at T-1; 1.00 ± 1.00 CFU/m at T0; 6.67 ± 6.03 CFU/m at T1; 10.00 ± 9.54 CFU/m at T2 and 4.33 ± 3.06 CFU/m at T3. At the OS in the HOT, the CFU were respectively 0.00 ± 0.00 at T0; 0.67 ± 0.58 at T1; 0.00 ± 0.00 at T2 and 0.00 ± 0.00 T3.
This standardized sterile technique in an OCR setting, with a mobile LAF, reduces the microbial colonies to levels that are safe and comparable to those recorded in the HOT setting, thus potentially improving the safety of IVIs in an OCR setting.
评估并比较在没有通风系统的玻璃体内注射（IVI）专用门诊洁净室（OCR）和医院手术室（HOT）中使用移动层流空气（LAF）设备时的分枝杆菌载量。
本病例对照研究在2种不同环境中进行：一系列玻璃体内注射期间的OCR和HOT。在两种环境下的玻璃体内注射过程中的三个不同时刻，使用表面空气系统仪器进行空气微生物分析：手术部位（OS）、距手术部位4米处（DOS）和消毒室（DR）。
在OCR的手术部位，T-1时微生物载量为20.33±16.51 CFU/m；T0时为1.00±1.00 CFU/m；T1时为6.67±6.03 CFU/m；T2时为10.00±9.54 CFU/m；T3时为4.33±3.06 CFU/m。在HOT的手术部位，CFU在T0时分别为0.00±0.00；T1时为0.67±0.58；T2时为0.00±0.00；T3时为0.00±0.00。
在OCR环境中采用这种配备移动LAF的标准化无菌技术，可将微生物菌落数量降低到安全水平，且与HOT环境中记录的水平相当，从而有可能提高OCR环境中玻璃体内注射的安全性。
