缺血性中风患者血清miR-142-5p升高与缺血性损伤以及NSE和S100β均相关。
Elevated serum miR-142-5p correlates with ischemic lesions and both NSE and S100β in ischemic stroke patients.
作者信息
Xu WeiWei, Cheng YongXia, An Ning, Jiang MeiLing
机构信息
Department of Neurology 3, Hongqi Hospital Affiliated to Mudanjiang Medical College, Mudanjiang, Heilongjiang, 157011, China.
Mudanjiang Medical College, Mudanjiang, Heilongjiang, 157011, China.
出版信息
Open Med (Wars). 2024 Dec 9;19(1):20241015. doi: 10.1515/med-2024-1015. eCollection 2024.
BACKGROUND
This study aims to evaluate the correlation between miRNAs and known nerve injury markers neuron-specific enolase (NSE) and S100β in ischemic stroke (IS) patients, exploring its efficacy.
METHODS
We retrospectively analyzed 86 IS patients and 32 healthy controls. Clinical and neurological examinations were performed in the admitted patients and the severity of neurological deficits was assessed by National Institutes of Health Stroke Scale (NIHSS). Plasma extraction and serum isolation were performed on all subjects before and 2 weeks after admission. miR-142-5p in serum, and NSE and S100β contents were measured by RT-qPCR and ELISA.
RESULTS
Ischemic lesions were more severe in IS patients, and NSE and S100β were abnormally elevated. miR-142-5p in the serum of IS patients was 2.85 times higher. After 2 weeks of treatment, serum miR-142-5, NSE, and S100β decreased. Patients' serum levels of miR-142-5p were 57.5% lower. Serum miR-142-5, NSE, and S100β were lower in patients with disease improvement than in patients with poor recovery. Additionally, miR-142-5 was positively correlated with NSE ( < 0.0001) and S100β ( = 0.0147), and also with the NIHSS score ( = 0.0004).
CONCLUSIONS
miR-142-5p, NSE, and S100β in peripheral blood (PB) of IS patients are elevated, and miR-142-5p is positively correlated with NSE and S100β.
背景
本研究旨在评估缺血性中风（IS）患者中微小RNA（miRNAs）与已知神经损伤标志物神经元特异性烯醇化酶（NSE）和S100β之间的相关性，并探索其有效性。
方法
我们回顾性分析了86例IS患者和32名健康对照者。对入院患者进行临床和神经学检查，并通过美国国立卫生研究院卒中量表（NIHSS）评估神经功能缺损的严重程度。在所有受试者入院前及入院后2周进行血浆提取和血清分离。采用逆转录定量聚合酶链反应（RT-qPCR）和酶联免疫吸附测定（ELISA）法检测血清中的miR-142-5p以及NSE和S100β的含量。
结果
IS患者的缺血性损伤更为严重，NSE和S100β异常升高。IS患者血清中的miR-142-5p升高了2.85倍。治疗2周后，血清miR-142-5、NSE和S100β水平下降。患者血清miR-142-5p水平降低了57.5%。病情改善患者的血清miR-142-5、NSE和S100β水平低于恢复较差的患者。此外，miR-142-5与NSE（<0.0001）和S100β（=0.0147）呈正相关，也与NIHSS评分（=0.0004）呈正相关。
结论
IS患者外周血（PB）中的miR-142-5p、NSE和S100β升高，且miR-142-5p与NSE和S100β呈正相关。
Zotero 插件