神经母细胞瘤的染色体分析。

Hafez M, el-Meadday M, Sheir M, Al-Tonbarry Y, Nada N, el-Desoky I

Br J Cancer. 1985 Feb;51(2):237-43. doi: 10.1038/bjc.1985.34.

Ten children with abdominal neuroblastoma were included in the study. Biopsies from the neuroblastomas were taken during surgical operations, nine from the primary tumours and one from a metastasis. Histopathology was done for diagnosis. Chromosomal cultures of neuroblastoma cells and peripheral blood lymphocytes were performed. The peripheral blood lymphocytes revealed normal chromosomal complements. The 10 tumours were in the peridiploid range with random gains or losses of chromosomes. Deletion of the short arm of chromosome number 1 distal to band p31 occurred in 6 tumours. Other structural changes and giant markers were found. It was concluded that a regulatory gene controlling the transformation gene of neuroblastoma, is present at or distal to lp31.

本研究纳入了10例腹部神经母细胞瘤患儿。在手术过程中对神经母细胞瘤进行活检，9例取自原发肿瘤，1例取自转移灶。进行组织病理学检查以明确诊断。对神经母细胞瘤细胞和外周血淋巴细胞进行染色体培养。外周血淋巴细胞显示染色体组正常。10个肿瘤处于亚二倍体范围，伴有染色体的随机增减。6个肿瘤发生了1号染色体短臂p31带远端的缺失。还发现了其他结构改变和巨大标记物。得出的结论是，控制神经母细胞瘤转化基因的调控基因位于lp31或其远端。