Sorrentino S, Lavitrano M, De Prisco R, Libonati M
Biochim Biophys Acta. 1985 Feb 4;827(2):135-9. doi: 10.1016/0167-4838(85)90081-0.
Human seminal ribonuclease (a basic protein occurring in a glycosylated and in a non-glycosylated form) is very active against double-stranded RNAs (De Prisco, R., Sorrentino, S., Leone, E. and Libonati, M. (1984) Biochim. Biophys. Acta 788, 356-363). The action of the two enzyme forms on single-stranded and double-stranded substrates was studied as a function of pH and ionic strength. Results indicate (1) that glycosylation of the RNAase molecule does not affect enzyme action on single-stranded RNAs, while (2) degradation of double-stranded RNAs is moderately increased by the presence of carbohydrates in the enzyme molecule. Human seminal RNAase shows a marked helix-destabilizing activity on poly(dA-dT) X poly(dA-dT). Under various conditions, this action (1) is definitely stronger than that of bovine RNAase A, and (2) seems to be less dependent on the glycosylation than on the basicity of the enzyme protein. The remarkable activity of human seminal RNAase on double-stranded RNA may, at least partly, be related to the enzyme properties mentioned above.
人精浆核糖核酸酶（一种以糖基化和非糖基化形式存在的碱性蛋白质）对双链RNA具有很高的活性（德普里斯科，R.，索伦蒂诺，S.，莱昂内，E.和利博纳蒂，M.（1984年）《生物化学与生物物理学报》788，356 - 363）。研究了两种酶形式对单链和双链底物的作用与pH值和离子强度的关系。结果表明：（1）核糖核酸酶分子的糖基化不影响其对单链RNA的作用，而（2）酶分子中碳水化合物的存在会适度增强双链RNA的降解。人精浆核糖核酸酶对聚（dA - dT）×聚（dA - dT）表现出显著的解螺旋活性。在各种条件下，这种作用：（1）肯定比牛核糖核酸酶A的作用更强，并且（2）似乎与其糖基化的相关性小于与酶蛋白碱性的相关性。人精浆核糖核酸酶对双链RNA的显著活性可能至少部分与上述酶的特性有关。
