Saraiva Juliane Torchelsen, Dos Santos Francieli da Silva, Bona Natália Pontes, da Silveira Larissa Menezes, Simões William Sanabria, da Silva Giulia Bueno de Oliveira, da Silva Júlia Araújo, Domingues William Borges, Nascimento Mariana Cavalcanti, Campos Vinicius Farias, Spanevello Roselia Maria, Pedra Nathalia Stark, Stefanello Francieli Moro
Programa de Pós-Graduação em Bioquímica e Bioprospecção - Laboratório de Biomarcadores, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário S/N, Pelotas, RS, CEP 96010-900, Brazil.
Programa de Pós-Graduação em Biotecnologia - Laboratório de Genômica Estrutural, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Campus Universitário S/N, Pelotas, RS, Brazil.
Neurochem Res. 2024 Dec 13;50(1):56. doi: 10.1007/s11064-024-04305-7.
Among the spectrum of gliomas, glioblastoma stands out as the most aggressive brain tumor affecting the central nervous system. In addressing this urgent medical challenge, exploring therapeutic alternatives becomes imperative to enhance the patient's prognosis. In this regard, Butia odorata (BO) fruit emerges as a promising candidate due to its array of bioactive compounds, including flavonoids, phenolic acids, and carotenoids, known for their antioxidant, anti-inflammatory, and antitumor properties. Thus, this study aimed to investigate the impact of standardized hydroalcoholic extract of BO on rat C6 and human U87MG glioma cell lines. Cells were exposed to varying extract concentrations (125-2000 μg/mL) for intervals of 0, 2, 4, 6, 24, 48, or 72 h. Then, cell viability, proliferation, colony formation, redox equilibrium parameters, cell migration, and the relative mRNA expression of genes related to gliomagenesis were evaluated. Our findings revealed a reduction in viability, proliferation, colony formation, reactive oxygen species, and nitrite levels in both glioma cell lines upon exposure to the extract. Conversely, an increase in sulfhydryl content and the activity of superoxide dismutase and catalase were observed in both glioma cell lines. No significant changes in viability and proliferation were observed in astrocytes. Furthermore, in the C6 cells only, the BO extract reduced the migration and downregulated the relative mRNA expression of matrix metalloproteinase-2, O6-methylguanine-DNA methyltransferase, nuclear factor-kappa B, interleukin-6 genes, and upregulated caspase-3 gene. These results underscore the promising anti-glioma potential of BO extract, attributed to its diverse bioactive composition.
在胶质瘤谱系中，胶质母细胞瘤是影响中枢神经系统的最具侵袭性的脑肿瘤。为应对这一紧迫的医学挑战，探索治疗方案对于改善患者预后至关重要。在这方面，香棕果因其一系列生物活性化合物（包括黄酮类、酚酸类和类胡萝卜素）而成为一个有前景的候选物，这些化合物以其抗氧化、抗炎和抗肿瘤特性而闻名。因此，本研究旨在调查标准化香棕水醇提取物对大鼠C6和人U87MG胶质瘤细胞系的影响。细胞暴露于不同浓度的提取物（125 - 2000μg/mL）中，时间间隔为0、2、4、6、24、48或72小时。然后，评估细胞活力、增殖、集落形成、氧化还原平衡参数、细胞迁移以及与胶质瘤发生相关基因的相对mRNA表达。我们的研究结果显示，两种胶质瘤细胞系在暴露于提取物后，活力、增殖、集落形成、活性氧和亚硝酸盐水平均降低。相反，在两种胶质瘤细胞系中均观察到巯基含量增加以及超氧化物歧化酶和过氧化氢酶活性增强。星形胶质细胞的活力和增殖未观察到显著变化。此外，仅在C6细胞中，香棕提取物减少了迁移，并下调了基质金属蛋白酶 - 2、O6 - 甲基鸟嘌呤 - DNA甲基转移酶、核因子 - κB、白细胞介素 - 6基因的相对mRNA表达，并上调了半胱天冬酶 - 3基因。这些结果强调了香棕提取物具有有前景的抗胶质瘤潜力，这归因于其多样的生物活性成分。
