Laboratório de Neuroquímica, Inflamação e Câncer, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário S/N, Pelotas, RS, CEP: 96010-900, Brazil.
Laboratório de Biomarcadores, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário S/N, Pelotas, RS, Brazil.
Metab Brain Dis. 2022 Feb;37(2):439-449. doi: 10.1007/s11011-021-00867-5. Epub 2021 Nov 8.
The aim of this study was to investigate the anticancer potential of blueberry extract (Vaccinium virgatum) against a C6 rat glioma lineage. Cultures of the C6 cells were exposed to blueberry extract at concentrations of 50 to 600 µg/mL for 12, 24, 48, or 72 h and then evaluated for cell viability, proliferation, migration, colony formation and oxidative stress. We also evaluated the effects of blueberry extract on primary rat cortical astrocytes. Our results show that treatment with blueberry extract did not alter the viability or proliferation of normal primary astrocytes but it did significantly reduce the viability in 21.54 % after 48 h and proliferation in 8.59 % after 24 h of C6 cells at 200 µg/mL. We also observed a reduction in the size of the colonies of 29.99 % at 100 µg/mL when compared to the control cells and cell migration was also reduced at 50 µg/mL. After 72 h, there was a reduction in the reactive oxygen species levels ranging from 46.26 to 34.73 %, in addition to a 380.2 % increase in total thiol content. Superoxide dismutase, catalase, and glutathione S-transferase activities were also enhanced when compared to the control. Taken together this data suggests that blueberry extract exerts some selective anticancer activity in C6 glioma cells.
本研究旨在探究蓝莓提取物（Vaccinium virgatum）对 C6 大鼠神经胶质瘤系的抗癌潜力。将 C6 细胞培养物暴露于 50 至 600μg/ml 的蓝莓提取物中 12、24、48 或 72 小时，然后评估细胞活力、增殖、迁移、集落形成和氧化应激。我们还评估了蓝莓提取物对原代大鼠皮质星形胶质细胞的影响。结果表明，蓝莓提取物处理不会改变正常原代星形胶质细胞的活力或增殖，但在 200μg/ml 时，C6 细胞的活力在 48 小时后显著降低了 21.54%，增殖在 24 小时后降低了 8.59%。我们还观察到，与对照细胞相比，100μg/ml 时集落的大小减少了 29.99%，而在 50μg/ml 时细胞迁移也减少了。72 小时后，活性氧水平从 46.26%降低到 34.73%，总巯基含量增加了 380.2%。与对照组相比，超氧化物歧化酶、过氧化氢酶和谷胱甘肽 S-转移酶的活性也增强了。总之，这些数据表明蓝莓提取物对 C6 神经胶质瘤细胞具有一定的选择性抗癌活性。
