Fries Alexander, Moldes Carlos Alberto, Mazzaferro Laura S
INCITAP-CONICET, FCEyN-Universidad Nacional de La Pampa (UNLPam), Santa Rosa, La Pampa, Argentina.
Nat Prod Res. 2024 Dec 13:1-9. doi: 10.1080/14786419.2024.2439024.
Cannabis oil, derived from plants, is increasingly used for therapeutic purposes across a wide range of diseases. Accurate quantification of cannabinoids is essential, especially for cannabis products sourced from informal markets where supply origins are uncertain. This study aimed to develop a cost-effective, robust analytical methodology using liquid chromatography in combination with UV- and mass detectors for the quantification of key cannabinoids (THC, CBD and CBN) and the identification of THCA and CBDA. Utilising an isocratic flow, the method achieved effective separation within 17 min, ensuring simplicity and reproducibility. The methodology validation was aligned with ICH guidelines' requirements for selectivity, linearity, precision, accuracy, and matrix effects. Successful application of this method to both homemade and commercial cannabis oil samples underscores its relevance for adjusting therapeutic doses and optimising CBD:THC ratios for specific disease treatments.
源自植物的大麻油越来越多地用于治疗多种疾病。准确量化大麻素至关重要，特别是对于来自供应来源不确定的非正式市场的大麻产品。本研究旨在开发一种具有成本效益、稳健的分析方法，使用液相色谱结合紫外和质谱检测器来量化关键大麻素（四氢大麻酚、大麻二酚和大麻酚）并鉴定四氢大麻酸和大麻二酚酸。该方法采用等度洗脱，在17分钟内实现了有效分离，确保了简单性和可重复性。方法验证符合国际人用药品注册技术协调会（ICH）指南对选择性、线性、精密度、准确度和基质效应的要求。该方法在自制和商业大麻油样品中的成功应用强调了其在调整治疗剂量和优化特定疾病治疗的大麻二酚：四氢大麻酚比例方面的相关性。
