开发并验证了一种简单、快速、易于使用的 HPLC-UV 方法，用于测定 L.提取物和药用油中的大麻素。

Development and Validation of a Simple, Fast, and Accessible HPLC-UV Method for Cannabinoids Determination in L. Extracts and Medicinal Oils.

机构信息

Facultad de Farmacia y Bioquímica, Instituto de Química y Metabolismo del Fármaco [IQUIMEFA], CONICET - Universidad de Buenos Aires, Junín 956, C.A. de Buenos Aires, Argentina.

Departamento de Farmacología, Cátedra de Farmacognosia, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, C.A. de Buenos Aires, Argentina.

出版信息

Curr Pharm Des. 2023;29(24):1918-1928. doi: 10.2174/1381612829666230809094304.

DOI:10.2174/1381612829666230809094304

PMID:37559239

Abstract

INTRODUCTION

L. is a well-recognized medicinal plant. Cannabis regulations in Argentina are insufficient to solve the problem of patient access to full-spectrum cannabis-based products. So, the market of artisanal products with unknown quality and dosage of cannabinoids is increasing, and so is the local demand and need for analyzing these products. However, much of the latest validated methodologies for cannabinoid quantification include expensive instrumentation that is not always available in laboratories of health institutions in Argentina.

METHODS

The aim of this work was to develop and validate a simple and rapid HPLC-UV method for the identification and quantification of principal cannabinoids in cannabis resins, inflorescences, and medicinal oils using standard HPLC equipment. The cannabinoids selected for validation were cannabidiol acid (CBDA), cannabigerol (CBG), cannabidiol (CBD), cannabinol (CBN), delta-9-tetrahydrocannabinol (Δ9-THC), cannabichromene (CBC), and tetrahydrocannabinol acid (THCA). A method for the simultaneous identification and quantification of these 7 main cannabinoids was developed and then validated. Some data parameters were comparable to other reports with more sophisticated analytical instruments for the analysis of cannabis. The assessed limits of detection and the limits of quantitation ranged from 0.9 to 3.66 μg/mL and 2.78 to 11.09 μg/mL, respectively. The concentration-response relationship of the method indicated a linear relationship between the concentration and peak area with R values of > 0.99 for all 7 cannabinoids.

RESULTS

The relative standard deviation (RSD%) varied from 2.34 to 4.82 for intraday repeatability and from 1.16 to 3.15 for interday repeatability. The percentage of recovery values was between 94 to 115% (resins) and 80 to 103% (inflorescence extract). The cannabis industry is growing rapidly, and there is a need for reliable testing methods to ensure the safety and efficacy of cannabis products. In addition, current methods for cannabinoid analysis are often time-consuming and expensive, while the HPLC-UV method herein reported is a simple, rapid, accurate, and cost-effective alternative for the analysis of cannabinoids in cannabis resins, inflorescences, and medicinal oils.

CONCLUSION

This method will be proposed to be included in the L. monograph of the Argentine Pharmacopoeia.

摘要

简介

L. 是一种广为人知的药用植物。阿根廷的大麻法规不足以解决患者获得全谱大麻素产品的问题。因此，质量和大麻素剂量未知的手工制作产品的市场正在扩大，当地对分析这些产品的需求也在增加。然而，最新的大麻素定量验证方法中的许多方法都包括昂贵的仪器，而阿根廷卫生机构的实验室并不总是具备这些仪器。

方法

本工作的目的是开发并验证一种简单快速的 HPLC-UV 方法，使用标准 HPLC 设备鉴定和定量分析大麻树脂、花序和药用油中的主要大麻素。选择用于验证的大麻素包括大麻二酚酸 (CBDA)、大麻萜酚 (CBG)、大麻二酚 (CBD)、大麻醇 (CBN)、Δ-9-四氢大麻酚 (Δ9-THC)、大麻色烯 (CBC) 和四氢大麻酸 (THCA)。开发了一种同时鉴定和定量分析这 7 种主要大麻素的方法，然后对其进行验证。一些数据参数与使用更复杂分析仪器分析大麻的其他报告相当。检测限和定量限的评估范围分别为 0.9 至 3.66 μg/mL 和 2.78 至 11.09 μg/mL。方法的浓度-响应关系表明，所有 7 种大麻素的浓度与峰面积之间呈线性关系，相关系数 (R 值) 均大于 0.99。

结果

日内重复性的相对标准偏差 (RSD%) 变化范围为 2.34%至 4.82%，日间重复性的 RSD% 变化范围为 1.16%至 3.15%。回收率值在 94%至 115%之间（树脂）和 80%至 103%之间（花序提取物）。大麻产业正在迅速发展，因此需要可靠的测试方法来确保大麻产品的安全性和有效性。此外，目前的大麻素分析方法通常既耗时又昂贵，而本文报告的 HPLC-UV 方法是一种简单、快速、准确且具有成本效益的替代方法，可用于分析大麻树脂、花序和药用油中的大麻素。