Kordbacheh Hamta, Katbab Ali Asghar, Aghvami-Panah Mohammad, Haghighipour Nooshin
Department of Polymer Engineering & Color Technology, Amirkabir University of Technology, Tehran, Iran.
Department of Polymer Engineering & Color Technology, Amirkabir University of Technology, Tehran, Iran.
Int J Biol Macromol. 2025 Feb;288:138681. doi: 10.1016/j.ijbiomac.2024.138681. Epub 2024 Dec 11.
This study presents the development of a novel piezoelectric scaffold for bone tissue engineering composed of poly(ε-caprolactone) (PCL), thermoplastic polyurethane (TPU), barium titanate (BT), and cellulose nanocrystals (CNC). PCL and TPU are considered advantageous materials because of their ease of processing, versatility in design, and ability to degrade over time; however, their inherent immiscibility poses challenges to achieving optimal porous structures. In this study, porous scaffolds were produced using gas foaming and salt leaching techniques, resulting in highly porous interconnected scaffolds exhibiting considerable elasticity that is suitable for dynamic cell culture while avoiding the use of toxic solvents. Given the piezoelectric nature of bone tissue, incorporating electric biosignals into scaffolds is essential to enhance bone regeneration. Therefore, BT was incorporated as a piezoelectric material. CNC, derived from cotton, assisted in BT distribution and acted as a reinforcing agent, imparting mechanoelectrical signaling properties to the scaffolds. The optimized scaffolds PCL/TPU (75/25) featuring 100 μm pores were integrated with varying BT and CNC ratios and were subjected to multiple analyses. The results showed a measurable electrical output of 1.2 mV and enhanced cell adhesion, viability, and proliferation under dynamic culture conditions, underscoring their potential for bone tissue regeneration.
本研究展示了一种用于骨组织工程的新型压电支架的开发，该支架由聚（ε-己内酯）（PCL）、热塑性聚氨酯（TPU）、钛酸钡（BT）和纤维素纳米晶体（CNC）组成。PCL和TPU因其易于加工、设计的多功能性以及随时间降解的能力而被认为是有利的材料；然而，它们固有的不相容性对实现最佳多孔结构构成了挑战。在本研究中，使用气体发泡和盐浸技术制备了多孔支架，得到了高度多孔的相互连接的支架，其具有相当大的弹性，适用于动态细胞培养，同时避免使用有毒溶剂。鉴于骨组织的压电性质，将电生物信号整合到支架中对于增强骨再生至关重要。因此，引入BT作为压电材料。源自棉花的CNC有助于BT的分布，并作为增强剂，赋予支架机电信号特性。优化后的具有100μm孔隙的PCL/TPU（75/25）支架与不同的BT和CNC比例相结合，并进行了多项分析。结果显示在动态培养条件下有1.2mV的可测量电输出，以及增强的细胞粘附、活力和增殖，突出了它们在骨组织再生方面的潜力。
