Heuck C C, Erbe I, Mathias D
Clin Chem. 1985 Feb;31(2):252-6.
We investigated the immunoprecipitation of apolipoprotein B-binding lipoproteins, or of apo A-I- and apo C-binding lipoproteins, by delipidated antiserum for measuring cholesterol in the nonprecipitated lipoprotein fractions. After immunoprecipitation of serum with delipidated anti-apo B, we determined by immunoelectrophoresis that no beta- or pre-beta lipoproteins were present, whereas alpha-lipoproteins remained in the supernate. Conversely, after immunoprecipitation with an antiserum against apo A-I + apo C, only lipoproteins with beta-mobility were detected and no apo B from beta-lipoproteins was in the precipitate. The concentration of cholesterol in the supernate after immunoprecipitation with anti-apo B correlated highly (r = 0.93, n = 118) with cholesterol measured after precipitation with phosphotungstic acid/MgCl2. The cholesterol concentration after immunoprecipitation with anti-apo AI and anti-apo C correlated similarly well (r = 0.94, n = 145) with the LDL-cholesterol calculated by Friedewald's formula in serum specimens reflecting moderate hyperlipoproteinemia.
我们研究了脱脂抗血清对载脂蛋白B结合脂蛋白或载脂蛋白A-I和载脂蛋白C结合脂蛋白的免疫沉淀作用，以测定未沉淀脂蛋白组分中的胆固醇。用脱脂抗载脂蛋白B血清对血清进行免疫沉淀后，我们通过免疫电泳确定不存在β脂蛋白或前β脂蛋白，而α脂蛋白保留在上清液中。相反，用抗载脂蛋白A-I + 载脂蛋白C抗血清进行免疫沉淀后，仅检测到具有β迁移率的脂蛋白，且沉淀中不存在来自β脂蛋白的载脂蛋白B。用抗载脂蛋白B免疫沉淀后上清液中的胆固醇浓度与用磷钨酸/MgCl2沉淀后测得的胆固醇高度相关（r = 0.93，n = 118）。在反映中度高脂蛋白血症的血清标本中，用抗载脂蛋白AI和抗载脂蛋白C免疫沉淀后的胆固醇浓度与用Friedewald公式计算的低密度脂蛋白胆固醇同样高度相关（r = 0.94，n = 145）。
