乙烯响应转录因子5（ERF5）增强了本氏烟草对烟草曲茎病毒及相关卫星β分子（TbCSV/TbCSB）的抗性。

Ethylene response transcription factor 5 (ERF5) enhances defense against tobacco curly shoot virus and associated betasatellite (TbCSV/TbCSB) in Nicotiana benthamiana.

作者信息

Zhao Meisheng, Zhang Liping, Ghanem Hussein, Wu Gentu, Li Mingjun, Qing Ling

机构信息

Chongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University, Chongqing, 400715, China.

Chongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University, Chongqing, 400715, China; Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), Southwest University, Chongqing, 400715, China.

出版信息

Virology. 2025 Feb;603:110309. doi: 10.1016/j.virol.2024.110309. Epub 2024 Nov 29.

DOI:10.1016/j.virol.2024.110309

Abstract

Begomovirus/betasatellite disease complex significantly threatens global crop production. Identifying potential plant antiviral genes is crucial for disease control. Nicotiana benthamiana is susceptible to viruses and contains 266 ethylene response transcription factors (ERFs). This study identified 29 NbERFs that were differentially upregulated in tobacco curly shoot virus and its associated betasatellite (TbCSV/TbCSB) infection, with ERF5 being the most common. Nine NbERF5s cluster phylogenetically and Niben101Scf00163g22002 (NbERF5) responds significantly to exogenous ACC treatment. Further analysis confirms the nuclear localization and transcriptional activation activity of NbERF5. Protein interaction assays demonstrate that NbERF5 has no self-interaction and does not interact with the βC1 protein of TbCSB. Silencing NbERF5 enhances TbCSV/TbCSB infection, and overexpression of NbERF5 inhibits TbCSV/TbCSB infection. Importantly, NbERF5 positively regulates the expression of the pathogenesis-related (PR) genes, NbPR1a and NbNPR1. Our findings suggest that NbERF5 enhances TbCSV/TbCSB resistance by activating the PR genes, indicating that NbERF5 is a potential antiviral gene.

摘要

双生病毒/β卫星病害复合体严重威胁全球作物生产。鉴定潜在的植物抗病毒基因对于病害控制至关重要。本氏烟草易受病毒感染，含有266个乙烯响应转录因子（ERF）。本研究鉴定出29个在烟草曲茎病毒及其相关β卫星（TbCSV/TbCSB）感染中差异上调的NbERF，其中ERF5最为常见。9个NbERF5在系统发育上聚类，且Niben101Scf00163g22002（NbERF5）对外源ACC处理有显著响应。进一步分析证实了NbERF5的核定位和转录激活活性。蛋白质相互作用分析表明，NbERF5没有自我相互作用，也不与TbCSB的βC1蛋白相互作用。沉默NbERF5会增强TbCSV/TbCSB感染，而过表达NbERF5则会抑制TbCSV/TbCSB感染。重要的是，NbERF5正向调节病程相关（PR）基因NbPR1a和NbNPR1的表达。我们的研究结果表明，NbERF5通过激活PR基因增强了对TbCSV/TbCSB的抗性，表明NbERF5是一个潜在的抗病毒基因。