Lin Jinhui, Chen Ting, Liu Xinming, Chen Zhen, Lei Yan
Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 350013, China.
Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 350013, China.
Int J Biol Macromol. 2025 Feb;288:138731. doi: 10.1016/j.ijbiomac.2024.138731. Epub 2024 Dec 16.
Grape (Vitis vinifera) production globally faces significant challenges from grape ripe rot (Colletotrichum viniferum). MYB transcription factors (TFs) play a crucial role in mediating plant responses to biotic stresses. However, their involvement in grapevine responses to ripe rot remains unclarified. This study employed bioinformatics to identify MYB TF genes within the grapevine genome and assessed their expression profiles post C. viniferum infection via transcriptome analysis. Among the 121 R2R3-MYB genes identified, VdMYB31, predominantly expressed in mature fruits, was notably downregulated in responding to C. viniferum infection. Heterologous expression of VdMYB31 in tomato (Solanum lycopersicum) fruits increased susceptibility to C. acutatum by suppressing salicylic acid (SA)-related gene expression. Silencing MYB31 in grape berries conferred resistance to C. viniferum. Additionally, exogenous SA application bolstered grape resistance to C. viniferum infection. These findings underscore the involvement of R2R3-MYB TFs in defending against C. viniferum, positioning VdMYB31 as a promising target for breeding grape varieties with improved resistance to ripe rot in viticulture.
全球葡萄（Vitis vinifera）生产面临着由葡萄炭疽病（Colletotrichum viniferum）带来的重大挑战。MYB转录因子（TFs）在介导植物对生物胁迫的反应中起着关键作用。然而，它们在葡萄对炭疽病的反应中的作用仍不明确。本研究利用生物信息学方法在葡萄基因组中鉴定MYB TF基因，并通过转录组分析评估它们在葡萄炭疽病菌感染后的表达谱。在鉴定出的121个R2R3-MYB基因中，主要在成熟果实中表达的VdMYB31在响应葡萄炭疽病菌感染时显著下调。VdMYB31在番茄（Solanum lycopersicum）果实中的异源表达通过抑制水杨酸（SA）相关基因的表达增加了对尖孢炭疽菌（C. acutatum）的易感性。在葡萄浆果中沉默MYB31赋予了对葡萄炭疽病菌的抗性。此外，外源施用SA增强了葡萄对葡萄炭疽病菌感染的抗性。这些发现强调了R2R3-MYB TFs在抵御葡萄炭疽病菌中的作用，将VdMYB31定位为葡萄栽培中培育抗炭疽病能力提高的葡萄品种的一个有前景的靶点。
