用于沙门氏菌分离株筛查和分组的API SerImm Sure试纸条评估

Evaluation of API SerImm Sure strips for screening and grouping Salmonella isolates.

作者信息

Evins G M, Linne L L, McWhorter A C

出版信息

Diagn Microbiol Infect Dis. 1985 Jan;3(1):19-23. doi: 10.1016/0732-8893(85)90062-8.

Abstract

Two new latex agglutination products in which the reagents are dried in macrocupules in plastic strips were evaluated. API SerImm Sure Salmonella Poly for screening and API SerImm Sure Salmonella for grouping (Analytab Products, Plainview, NY) were tested with 63 recent isolates and 72 stock strains of Salmonella groups A-E and 43 stock strains of Salmonella groups F-67. Of the homologous strains, the polyvalent reagent correctly identified 89%, and the grouping reagents 90%. The most commonly occurring serotypes of groups A-E were identified. The major problems were failure to identify Group B 1,4,12,27, and group D29,46 strains, and lack of macrocupules with normal serum-latex controls. The major advantage of the products is convenience, since the SerImm Sure packaging eliminated the need to dilute anti-sera and prepare slides.

摘要

对两种新型乳胶凝集产品进行了评估，其试剂被干燥在塑料条的大孔中。用于筛选的API SerImm Sure沙门氏菌多价试剂和用于分组的API SerImm Sure沙门氏菌试剂（Analytab Products公司，纽约州普莱恩维尤），用63株近期分离株以及A - E组沙门氏菌的72株标准菌株和F - 67组沙门氏菌的43株标准菌株进行了测试。在同源菌株中，多价试剂正确鉴定出89%，分组试剂正确鉴定出90%。A - E组最常见的血清型被鉴定出来。主要问题是未能鉴定出B组1,4,12,27菌株和D组29,46菌株，以及正常血清 - 乳胶对照缺乏大孔。这些产品的主要优点是方便，因为SerImm Sure包装无需稀释抗血清和制备玻片。