Chu Wei, Pan Hongqiu, Fei Zhongting, Zhang Tiantian
Tuberculosis Department, The Third People's Hospital of Zhenjiang, Zhenjiang, 212000, Jiangsu Province, China.
Tuberculosis Department, Huai'an No.4 People's Hospital, Huaian, 223000, Jiangsu Province, China.
J Infect Chemother. 2025 Apr;31(4):102589. doi: 10.1016/j.jiac.2024.12.018. Epub 2024 Dec 13.
This study sought to investigate the diagnostic value and the effect of microRNA (miR)-206 on drug resistance in pulmonary tuberculosis (TB) patients.
This study included 88 TB patients (TB group) as study subjects, 80 healthy subjects as control 1 (Control group), and 85 latent TB infection (LTBI) patients as control 2 (LTBI group). The drug resistance of TB patients after standard anti-TB treatment was recorded. TB patients were assigned into the pan-sensitive and drug-resistance groups, with miR-206 level in drug-resistant TB patients analyzed. The correlation coefficients between inflammatory indicators (TNF-α, IgG, IL-4, IFN-γ, IP-10) and drug resistance in TB patients were analyzed, and the independent correlation between miR-206 levels and drug resistance was analyzed.
Compared to the Control and LTBI groups, serum miR-206 and IP-10 were highly-expressed in TB group. The miR-206 levels positively correlated with IP-10 levels. miR-206 had potential diagnostic value for TB. Levels of TNF-α, IgG, IFN-γ, and IL-4 were elevated in TB group and positively-correlated with miR-206 levels. Moreover, miR-206 levels were higher in the Drug-resistance group than the pan-sensitive group. The low-expression group had a lower incidence of drug resistance than the high-expression group (χ = 16.84, P < 0.0001). miR-206 was the most significant indicator affecting drug resistance in TB patients (β = 0.516, P = 0.013). miR-206 was an independent risk factor for drug resistance.
High miR-206 expression helps TB diagnosis and may predict drug-resistance incidence. miR-206 may be an independent risk factor for drug resistance in TB patients.
本研究旨在探讨微小RNA（miR）-206对肺结核（TB）患者的诊断价值及对耐药性的影响。
本研究纳入88例TB患者（TB组）作为研究对象，80例健康受试者作为对照组1（对照组），85例潜伏性结核感染（LTBI）患者作为对照组2（LTBI组）。记录TB患者标准抗结核治疗后的耐药情况。将TB患者分为全敏感组和耐药组，分析耐药TB患者的miR-206水平。分析炎症指标（TNF-α、IgG、IL-4、IFN-γ、IP-10）与TB患者耐药性之间的相关系数，并分析miR-206水平与耐药性之间的独立相关性。
与对照组和LTBI组相比，TB组血清miR-206和IP-10高表达。miR-206水平与IP-10水平呈正相关。miR-206对TB具有潜在诊断价值。TB组TNF-α、IgG、IFN-γ和IL-4水平升高，且与miR-206水平呈正相关。此外，耐药组的miR-206水平高于全敏感组。低表达组的耐药发生率低于高表达组（χ = 16.84，P <
0.0001）。miR-206是影响TB患者耐药性的最显著指标（β = 0.516，P = 0.013）。miR-206是耐药的独立危险因素。
miR-206高表达有助于TB诊断，并可能预测耐药发生率。miR-206可能是TB患者耐药的独立危险因素。
