miRNA-378 表达水平高对肺结核具有较高的诊断价值，并可预测不良预后。

High miRNA-378 expression has high diagnostic values for pulmonary tuberculosis and predicts adverse outcomes.

机构信息

Department of Tuberculosis Treatmen and Prevention, Shaanxi Provincial Institute for Tuberculosis Control and Prevention, 4# of Xingqing South Road, Xi'an, 710048, Shaanxi, China.

Department of Orthopedics, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

出版信息

BMC Mol Cell Biol. 2022 Mar 19;23(1):14. doi: 10.1186/s12860-022-00413-w.

DOI:10.1186/s12860-022-00413-w

PMID:35305574

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8934448/

Abstract

BACKGROUND

Pulmonary tuberculosis (TB) is a chronic infectious disease. microRNA (miR)-378 is involved in TB diagnosis. This study explored the effects of miR-378 on TB patients.

METHODS

A total of 126 TB patients were selected, including 63 active TB and 63 latent TB, with 62 healthy subjects as controls. Serum miR-378 expression was detected. The diagnostic value of miR-378 in TB was analyzed using the ROC curve. Immune inflammatory factor levels were detected and their correlations with miR-378 expression were analyzed. The drug resistance of active TB patients was recorded after standard treatment. miR-378 expression in drug-resistant TB patients was detected. The effects of miR-378 on adverse outcome incidence were analyzed.

RESULTS

miR-378 expression was highly expressed in TB and the expression was higher in the active group than the latent group. Serum miR-378 expression > 1.490 had high sensitivity and specificity in TB diagnosis. miR-378 expression was correlated with TB clinical indexes. IL-4, IL-6, and IL-1β levels were highly expressed, while IFN-γ, TNF-α, and IL-12 levels were lowly expressed in TB patients. Serum miR-378 level in the active group was positively correlated with serum IL-4, IL-6, and IL-1β, and negatively correlated with serum IFN-γ, TNF-α, and IL-12 concentrations. miR-378 expression was downregulated in the TB treated, single (SDR TB) and multi-drug resistance (MDR TB) groups, the miR-378 expression in SDR TB and MDR TB groups was higher than the TB treated group and lower in the SDR TB group than the MDR TB group. High miR-378 expression predicted higher adverse outcome incidence.

CONCLUSIONS

High miR-378 expression assisted TB diagnosis and predicted adverse outcomes.

摘要

背景

肺结核（TB）是一种慢性传染病。microRNA（miR）-378 参与了 TB 的诊断。本研究探讨了 miR-378 对 TB 患者的影响。

方法

选取 126 例 TB 患者，包括 63 例活动性 TB 和 63 例潜伏性 TB，以 62 例健康受试者作为对照。检测血清 miR-378 表达水平。采用 ROC 曲线分析 miR-378 在 TB 中的诊断价值。检测免疫炎症因子水平，并分析其与 miR-378 表达的相关性。对活动性 TB 患者经标准治疗后记录耐药情况。检测耐药性 TB 患者的 miR-378 表达水平。分析 miR-378 对不良预后发生率的影响。

结果

TB 患者 miR-378 表达水平升高，且活动组高于潜伏组。血清 miR-378 表达量>1.490 时对 TB 诊断具有较高的灵敏度和特异性。miR-378 表达与 TB 临床指标相关。TB 患者 IL-4、IL-6、IL-1β 水平高表达，IFN-γ、TNF-α、IL-12 水平低表达。活动组血清 miR-378 水平与血清 IL-4、IL-6、IL-1β 呈正相关，与血清 IFN-γ、TNF-α、IL-12 浓度呈负相关。经治疗后、单耐药（SDR TB）和耐多药（MDR TB）组的 miR-378 表达均下调，SDR TB 和 MDR TB 组的 miR-378 表达高于治疗组，SDR TB 组低于 MDR TB 组。高 miR-378 表达预测不良预后发生率较高。