Tran Thi Ngoc Tran, Tran Quang Minh, Le Ngoc Ha-Thu
Faculty of Materials Science and Technology, University of Science, VNU-HCM, 700000, Viet Nam; Viet Nam National University, Ho Chi Minh City, 700000, Viet Nam.
Faculty of Materials Science and Technology, University of Science, VNU-HCM, 700000, Viet Nam; Viet Nam National University, Ho Chi Minh City, 700000, Viet Nam.
Int J Biol Macromol. 2025 Feb;289:138768. doi: 10.1016/j.ijbiomac.2024.138768. Epub 2024 Dec 13.
This study aimed to prepare Piper betle L. extract-load chitosan/polyvinyl alcohol (CS/PVA) film potential for wound dressing and investigate the effects of PLE and PLE-loading methods on physicochemical and biological properties of CS/PVA films. First, Piper betle L. extract (PLE) was optimized using ultrasonication and the response surface methodology employed the Box-Behnken design to maximize total phenolic content (TPC), total flavonoid content (TFC), and natural antioxidant activity. The optimal ultrasonic conditions resulting in an extract yield of 17.466 %, TPC of 261.904 mg GA/g, TFC of 148.726 mg Q/g, and IC of 53.100 mg/L were achieved with a sonication time of 3.958 min, power of 30.548 W, and duty cycle of 84.576 % using water as the green solvent. The systematic analysis explored the effects of extraction duration, power, and pulse mode providing valuable insights into novel extraction techniques for potential pharmaceutical applications. Subsequently, PLE was incorporated into a CS/PVA biocomposite film using two loading methods: direct mixing and immersion. The study revealed that the immersion method offers several advantages related to the physicochemical and biological properties of the PLE-treated CS/PVA film. These advantages include improved PLE bioavailability (with PLE releasing 81.42 ± 2.44 % over 24 h, 8.6 times higher than the direct mixing method), removal of excess acetic acid from the manufacturing process of CS/PVA film, which causes cell cytotoxicity (L929 cell viability of 70.47 ± 2.18 %), enhanced tensile strength of 1.19 times greater than the original CS/PVA film, and efficient exudate absorption (allowing appropriate water vapor transmission at a rate of 2477.00 ± 35.39 g/m·day). The results show the prepared PLE-treated CS/PVA film is a potential candidate for wound dressing, and the immersion method represents an advanced drug-loading method, especially for medicinal herbs on CS/PVA thin film surfaces.
本研究旨在制备具有伤口敷料潜力的蒌叶提取物负载壳聚糖/聚乙烯醇（CS/PVA）薄膜，并研究蒌叶提取物（PLE）及其负载方法对CS/PVA薄膜物理化学和生物学性质的影响。首先，采用超声处理优化蒌叶提取物（PLE），并使用Box-Behnken设计的响应面法来最大化总酚含量（TPC）、总黄酮含量（TFC）和天然抗氧化活性。以水作为绿色溶剂，在超声时间为3.958分钟、功率为30.548瓦、占空比为84.576%的条件下，获得了最佳超声条件，提取物产率为17.466%，TPC为261.904毫克没食子酸/克，TFC为148.726毫克芦丁/克，IC为53.100毫克/升。系统分析探讨了提取时间、功率和脉冲模式的影响，为潜在药物应用的新型提取技术提供了有价值的见解。随后，采用两种负载方法将PLE掺入CS/PVA生物复合薄膜中：直接混合和浸泡。研究表明，浸泡法在与PLE处理的CS/PVA薄膜的物理化学和生物学性质相关方面具有若干优势。这些优势包括提高PLE生物利用度（PLE在24小时内释放81.42±2.44%，比直接混合法高8.6倍）、去除CS/PVA薄膜制造过程中导致细胞毒性的过量乙酸（L929细胞活力为70.47±2.18%）、拉伸强度比原始CS/PVA薄膜提高1.19倍以及有效吸收渗出液（允许以2477.00±35.39克/平方米·天的速率进行适当的水蒸气透过）。结果表明，制备的PLE处理的CS/PVA薄膜是伤口敷料的潜在候选材料，浸泡法是一种先进的载药方法，尤其适用于CS/PVA薄膜表面的草药。
