Ghaemi Mehran, Sadeghi Mohammad, Kafi Mojtaba, Pakniat Zohreh
Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Vet Res Commun. 2024 Dec 16;49(1):51. doi: 10.1007/s11259-024-10617-4.
A remarkable percentage of cows in industrialized dairy farms can be affected by uterine diseases including endometritis. Different viral and bacterial agents are causing endometritis in dairy cows including Trueperella pyogenes, and bovine alphaherpesvirus 1. Also, bovine gammaherpesvirus type 4 (BoGHV-4) is responsible for different reproductive disorders. The present study aimed to investigate the role of BoGHV-4 in histopathological and cytological changes of post-partum uterine diseases and its relation to bacterial co-infection. Forty-seven uteri were collected from Holstein dairy cows at least 35 days post-partum. Bacterial culture, cytological, and histopathological studies were conducted on the endometrial samples. The PCR reaction was conducted for the sequencing of the gB gene. PCR examination indicated that from 47 uteri obtained from a slaughterhouse, 13 uteri (27.7%) were positive for BoGHV-4. Statistical analysis revealed a significant relationship between virus presence, and histopathologic and cytologic endometritis (P < 0.05). Also, Escherichia coli and Fusobacterium necrophorum presence was significantly related to BoGHV-4 infection. Vast varieties of other bacterial species were isolated from different samples but the relation between these bacteria and BoGHV-4 was not significant (P > 0.05). Altogether, it can be concluded that BoGHV-4 can play some roles in establishing endometrial inflammation increasing leukocyte infiltration in endometrium, and enhancing bacterial infection in the uterus of dairy cows. This is the first study, which detected the BoGHV-4 in Iranian cows using molecular methods.
工业化奶牛场中有相当比例的奶牛会受到包括子宫内膜炎在内的子宫疾病影响。不同的病毒和细菌病原体可导致奶牛子宫内膜炎，包括化脓隐秘杆菌和牛α疱疹病毒1。此外，牛γ疱疹病毒4型（BoGHV - 4）可引发不同的生殖障碍。本研究旨在探讨BoGHV - 4在产后子宫疾病的组织病理学和细胞学变化中的作用及其与细菌合并感染的关系。从产后至少35天的荷斯坦奶牛收集了47个子宫。对子宫内膜样本进行细菌培养、细胞学和组织病理学研究。进行PCR反应以对gB基因进行测序。PCR检测表明，从屠宰场获得的47个子宫中，有13个子宫（27.7%）BoGHV - 4呈阳性。统计分析显示病毒存在与组织病理学和细胞学子宫内膜炎之间存在显著关系（P < 0.05）。此外，大肠杆菌和坏死梭杆菌的存在与BoGHV - 4感染显著相关。从不同样本中分离出了大量其他细菌种类，但这些细菌与BoGHV - 4之间的关系不显著（P > 0.05）。总之，可以得出结论，BoGHV - 4在奶牛子宫内膜炎症的发生、子宫内膜白细胞浸润增加以及子宫细菌感染增强方面可能发挥一些作用。这是第一项使用分子方法在伊朗奶牛中检测到BoGHV - 4的研究。
