Benchimol M, De Souza W
Exp Parasitol. 1985 Feb;59(1):51-8. doi: 10.1016/0014-4894(85)90056-6.
The zinc iodide-osmium tetroxide technique was used to analyze the distribution of the endoplasmic reticulum-Golgi complex system of Tritrichomonas foetus. Interconnections between the cisternae of the endoplasmic reticulum as well as between cisternae of the Golgi complex were observed. The nuclear pores, as well as fenestrations in the Golgi complex, were evident. Three to four profiles of the endoplasmic reticulum were seen in the proximal marginal lamellae, related to the attachment of the recurrent flagellum to the protozoan body. No reaction product was seen in the costae, microtubules, glycogen particles, or hydrogenosomes. Imidazole-buffered osmium tetroxide solution was used to visualize lipids. Electron-dense materials were seen either free in the cytoplasm or within membrane-bounded cytoplasmic vesicles. A high contrast of some membranes, mainly of those which enclosed the hydrogenosomes, was observed in unstained sections.
采用碘化锌-四氧化锇技术分析胎儿三毛滴虫内质网-高尔基体复合系统的分布。观察到内质网池之间以及高尔基复合体池之间的相互连接。核孔以及高尔基复合体中的窗孔很明显。在近端边缘板中可见三到四层内质网，与复鞭毛附着于原生动物体有关。在肋、微管、糖原颗粒或氢化酶体中未见反应产物。用咪唑缓冲的四氧化锇溶液使脂质可视化。电子致密物质可见于细胞质中游离状态或膜结合的细胞质小泡内。在未染色的切片中观察到一些膜，主要是包围氢化酶体的膜，具有高对比度。
